云南民族大学学报（自然科学版）2026，Vol.35Issue(3)：351-362,12.DOI:10.3969/j.issn.1672-8513.2026.03.008
α-葡萄糖苷酶抑制剂筛选方法的构建及应用
Establishment and application of a screening method for α-glucosidase inhibitors
摘要
Abstract
To rapidly discover lead compounds with α-glucosidase inhibitory activity from natural products for the treatment of type Ⅱ diabetes,this study established and validated an efficient screening platform based on ultraviolet spectrophotometry.Through systematic optimization,the optimal conditions were determined as follows:detection wavelength of 394 nm,200 μL of substrate(2 mg/mL),100 μL of enzyme(2 000 U/mL),100 μL of PBS buffer,a temperature of 37 ℃,and a reaction time of 300 s.Using this optimized system,the inhibitory activities of 206 aqueous extracts of traditional Chinese medicines(TCMs)were screened,with acarbose as the positive control.The results revealed that 28 TCM extracts exhibited inhibition rates exceeding 30％.Among them,the inhibition rates of Mulberry Bark(Morus alba L.),Silkworm Frass(Bombyx mori L.),and Honey-Processed Mulberry Bark all surpassed 60％,with IC50 values comparable to that of acarbose.This study demonstrates that the established system is straightforward,sensitive,and reliable,providing not only an experimental basis for the rapid screening of α-glucosidase inhibitors but also a feasible approach for discovering novel anti-diabetic active components from TCMs.关键词
Ⅱ型糖尿病/α-葡萄糖苷酶/抑制剂/筛选方法/中药Key words
type diabetes/α-glucosidase/inhibitor/screening method/traditional Chinese medicine分类
医药卫生引用本文复制引用
苟强强,冯再平,张新国,石琴翠,杜文韬,秦娜娜,王文娣,赵雪,徐佳楠,Omer Hassan Ali Hassan,王波..α-葡萄糖苷酶抑制剂筛选方法的构建及应用[J].云南民族大学学报（自然科学版）,2026,35(3):351-362,12.基金项目
国家自然科学基金(32160021) （32160021）
甘肃省重点研发计划(22YF7NA176) （22YF7NA176）
甘肃省自然科学基金(23JRRA792). （23JRRA792）
