云南民族大学学报（自然科学版）2026，Vol.35Issue(3)：371-378,8.DOI:10.3969/j.issn.1672-8513.2026.03.010
HPLC-RID法测定新型冠状病毒mRNA疫苗中蔗糖的质量浓度
HPLC-RID method for the determination of sucrose mass fraction in mRNA-based COVID-19 vaccines
摘要
Abstract
An HPLC-RID method for determining the sucrose mass fraction in COVID-19 mRNA vaccines was established using an aminopropyl-bonded silica column(4.6 mm × 250 mm,5.0 μm)with an acetonitrile-water solution as the mobile phase.Optimization was performed on the mobile phase ratio,flow rate,and column temperature.The method demonstrated a good linear relationship within the sucrose mass concentration range of 5.05-30.30 mg/mL,along with satisfactory specificity,repeatability,accuracy,and durability.Both the limit of quantification(LOQ)and limit of detection(LOD)met the analytical requirements.This approach not only provides a reliable analytical means for quantifying sucrose in COVID-19 mRNA vaccines but also establishes a research foundation for sucrose determination in other mRNA-based vaccines.关键词
蔗糖质量浓度/高效液相色谱法/示差折光检测器/新型冠状病毒mRNA疫苗Key words
sucrose mass fraction/high-performance liquid chromatography/refractive index detector/mRNA-based COVID-19 vaccines分类
医药卫生引用本文复制引用
刘勤,申雪,杨欢,非成瑞,王思捷,和文志,吴凡..HPLC-RID法测定新型冠状病毒mRNA疫苗中蔗糖的质量浓度[J].云南民族大学学报（自然科学版）,2026,35(3):371-378,8.基金项目
云南省科技厅重大科技专项计划(202002AA100008). （202002AA100008）
