现代畜牧科技Issue(6)：17-20,4.DOI:10.19369/j.cnki.2095-9737.2026.06.005
不同固定与染色条件对公牛精液抹片效果的影响研究
Effects of Different Fixation and Staining Conditions on Bull Semen Smear Quality
摘要
Abstract
This study aimed to optimize semen smear preparation methods for sperm morphological abnormality assessment,thereby improving the accuracy and efficiency of detection.By comparing fixation times(0.5,1,2,and 3 min),staining times(1,2,3,and 5 min),semen volumes(5,8,and 10 μL),and staining reagents(gentian violet and crystal violet),the effects of these parameters on smear background intensity and sperm staining quality were assessed.The results showed that smears prepared with 5 μL semen yielded better outcomes than those prepared with 8 μL and 10 μL.At this volume,gentian violet produced superior staining effects compared with crystal violet.Fixation for 3 min combined with staining for 3 or 5 min resulted in darker backgrounds,while fixation for 0.5 min led to insufficient staining of spermatozoa.Considering both staining quality and time efficiency,fixation for 1～2 min and staining for 1～2 min were identified as the optimal parameter range.Ultimately,use 5 μL of semen,fixation for 1 min with 95%ethanol and staining with 0.1%gentian violet for 1 min was determined to be the optimal combination,producing smears with clear backgrounds and well-defined sperm morphology.This protocol meets the requirements for sperm abnormality assessment and has considerable potential for practical application.关键词
精子畸形率/精液/固定/染色/精子形态Key words
sperm malformation rate/semen/fixation/staining/sperm morphology分类
农业科技引用本文复制引用
哈懿文,余伟荣,李章莲,俸正涛,陈红艳,杨方晓,赵若含,钱林东..不同固定与染色条件对公牛精液抹片效果的影响研究[J].现代畜牧科技,2026,(6):17-20,4.基金项目
云南农业职业技术学院重点项目(2024YNVC-ZD01) （2024YNVC-ZD01）
