现代畜牧科技Issue(7)：1-4,4.DOI:10.19369/j.cnki.2095-9737.2026.07.001
犬瘟热病毒RT-LAMP检测方法的建立及与RT-PCR检测方法比较分析
Establishment of RT-LAMP Method for Detection of Canine Distemper Virus and Comparative Analysis with RT-PCR Detection Method
摘要
Abstract
In order to establish a rapid clinical detection method for canine distemper virus,RT-LAMP internal primers,external primers and loop primer were designed for the study based on the conserved sequences of N gene of canine distemper virus.RT-LAMP assay was established by optimization of reaction conditions,sensitivity test,specificity test and clinical samples test.The results showed that the optimal reaction temperature was 63℃,and the lowest detectable plasmid concentration was 5.68×101 copies/μL.Combined with the clinical sample verification test,the established CDV RT-LAMP detection method had high specificity and higher sensitivity than the national standard RT-PCR detection method.It can be applied to the rapid clinical diagnosis of canine distemper and provides technical support for the next step of technology promotion and canine distemper prevention and control.关键词
犬瘟热/RT-LAMP/敏感性/检测Key words
canine distemper/RT-LAMP/sensitivity/detection分类
农业科技引用本文复制引用
张启龙,张玮,高晓龙,吴迪,王林..犬瘟热病毒RT-LAMP检测方法的建立及与RT-PCR检测方法比较分析[J].现代畜牧科技,2026,(7):1-4,4.基金项目
北京市乡村振兴农业科技项目(NY2502180025) （NY2502180025）
