现代畜牧科技Issue(7)：12-15,4.DOI:10.19369/j.cnki.2095-9737.2026.07.004
牛传染性鼻气管炎病毒微滴式数字PCR检测方法的建立
Development of a Droplet Digital PCR for Detection of Infectious Bovine Rhinotracheitis Virus
摘要
Abstract
To establish a detection method for IBRV with high sensitivity,high specificity,and absolute quantification capability,this study designed specific primers and probes targeting the gB gene of IBRV,thereby developing an IBRV droplet digital PCR(ddPCR)assay.Critical reaction parameters including annealing temperature and primer/probe concentrations were systematically optimized,followed by validation of the method's specificity,sensitivity,and concordance.The results demonstrated that:the assay exhibited excellent specificity,specifically detecting IBRV without any cross-reactivity with FMDV,BVDV,BRSV,BPIV 3.It showed high sensitivity,with a minimum detection limit as low as 0.94 copies/μL.It achieved a 100%concordance rate with the standard qPCR detection method,indicating high consistency.These findings confirm that the established IBRV ddPCR assay,characterized by strong specificity,high sensitivity,and excellent concordance,can meet the requirements for precise quantification of IBRV,early diagnosis,vaccine antigen quantification,import-export quarantine,and epidemiological surveillance.It thus provides technical support for the accurate detection of Infectious Bovine Rhinotracheitis.关键词
牛传染性鼻气管炎/绝对定量/微滴式数字PCRKey words
infectious bovine rhinotracheitis/absolute quantification/droplet digital PCR分类
农业科技引用本文复制引用
高晓龙,王林,梅力,高敏,栗云鹏,雷琪莉,张启龙,傅彩霞,赵浩军,冯小宇..牛传染性鼻气管炎病毒微滴式数字PCR检测方法的建立[J].现代畜牧科技,2026,(7):12-15,4.基金项目
北京市动物疫病预防控制中心综合保障项目:实验室运转与动物疫病监测(11000024Y000002861241) （11000024Y000002861241）
