养殖与饲料2026，Vol.25Issue(6)：46-51,6.DOI:10.13300/j.cnki.cn42-1648/s.2026.06.010
16S rRNA基因在3只华南兔残体鉴定上的应用
Identifying remains of three Lepus sinensis with sequencing 16S rRNA gene
刘继华 1王坤 2李鑫 3邹梅 4张华伟 5张斌 6杨志兵 7陈海龙8
作者信息
- 1. 郑州市动物园(郑州市犬只收容站),郑州 450008
- 2. 郸城县林业保护中心,河南 周口 477150
- 3. 南乐县林业发展中心,河南 濮阳 457400
- 4. 新蔡县佛阁寺镇行政审批服务中心,河南 驻马店 463500
- 5. 开封市公园服务中心,河南 开封 475000
- 6. 洛阳市偃师区林业技术推广站,河南 洛阳 471900
- 7. 郑州市森林公园,郑州 450000
- 8. 国有信阳市南湾林场,河南 信阳 464000
- 折叠
摘要
Abstract
[Objectives]To address the problems of identifying species of wild animal remains involved in legal cases,and to provide standardized technical references for wildlife forensic identification.[Methods]The 16S rRNA gene sequencing technology was used to analyze 3 samples of animal remains from a wild hare traf-ficking case in Shitou Township,Huixian City,Henan Province in December 2023.The species of the samples were identified via DNA extraction,PCR amplification,sequencing,sequence alignment,combined with ana-lyzing genetic distance and constructing phylogenetic trees.[Results]The similarity between the sequences of 16S rRNA gene in the 3 wild hare samples and Lepus sinensis was over 98%,and clustered into one branch in the phylogenetic tree with a support rate of 98%.The species of the 3 samples was clearly identified to be Lepus sinensis.The amount of money involved in the case was calculated to be 240 RMB according to relevant standards.[Conclusions]The 16S rRNA gene sequencing technology was used to accurately and effectively iden-tify species from remains of Lepus sinensis in this study,indicating that this technology is feasible for forensic identification of wildlife remains.关键词
华南兔/16S rRNA基因测序/物种鉴定/遗传距离/系统发育树Key words
Lepus sinensis/16S rRNA gene sequencing/species identification/genetic distance/phyloge-netic tree引用本文复制引用
刘继华,王坤,李鑫,邹梅,张华伟,张斌,杨志兵,陈海龙..16S rRNA基因在3只华南兔残体鉴定上的应用[J].养殖与饲料,2026,25(6):46-51,6.
