中国动物检疫2026，Vol.43Issue(5)：56-66,11.DOI:10.3969/j.issn.1005-944X.2026.05.008
CRISPR/Cas系统在动物疫病诊断中的应用
Applications of CRISPR/Cas Systems in Animal Disease Diagnosis
摘要
Abstract
As an emerging molecular diagnostic tool,clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)system has been developed into a novel technology for rapid detection of nucleic acids,benefiting from its advantages such as high sensitivity,high specificity,accessibility and suitability for on-site testing.Here,the CRISPR/Cas system was summarized,including its basic principles,immune mechanisms and classification.The characteristics of its three effector proteins,including Cas9,Cas 12 and Cas 13,and their applications in the detection of animal diseases were clarified.An integrated single-or multi-plex amplification and detection system was formed by the integration of different CRISPR/Cas systems with various isothermal nucleic acid amplification techniques,which was described in this review.It was demonstrated that,by numerous studies,the system was highly specific and sensitive,time-saving and free from complex instruments,and had been successfully applied for the simultaneous detection of multiple pathogens,as well as for typing and identification of same pathogen species,thereby demonstrating capability of precise detection.In addition,current challenges and future directions of the CRISPR/Cas system were also discussed.The application of CRISPR/Cas technology in animal disease diagnosis was reviewed,aiming to offer insights and references for future development of rapid,accurate and low-cost detection methods suitable for field application.关键词
CRISPR/Cas系统/动物疫病/检测技术/田间应用Key words
CRISPR/Cas system/animal disease/detection technology/field application分类
农业科技引用本文复制引用
聂健鸿,张皓博,孙翔翔,刘蒙达,樊晓旭,邵卫星,温建新,孙芳静,孙明军,焉鑫,张培培,亓菲,张研博,孙世雄,南文龙..CRISPR/Cas系统在动物疫病诊断中的应用[J].中国动物检疫,2026,43(5):56-66,11.基金项目
青岛市科技惠民示范专项(23-2-8-xdny-14-nsh,24-1-8-xdny-4-nsh) （23-2-8-xdny-14-nsh,24-1-8-xdny-4-nsh）
"十四五"国家重点研发计划项目(2025YFD1800102) （2025YFD1800102）
