中国临床药理学与治疗学2026，Vol.31Issue(5)：577-584,8.DOI:10.12092/j.issn.1009-2501.2026.05.001
益气活络方通过调控JAK2/STAT3通路对类风湿关节炎模型大鼠Th17/Treg平衡的影响
Effect of Yiqi Huoluo Formula on Th17/Treg balance in rheumatoid arthritis model rats via regulating the JAK2/STAT3 pathway
摘要
Abstract
AIM:To investigate the therapeutic ef-fect of Yiqi Huoluo Formula on collagen-induced arthritis(CIA)rat models and its influence on the Th17/Treg balance by regulating the JAK2/STAT3 pathway.METHODS:The CIA rat models were es-tablished using bovine type II collagen emulsion in-duction,with tofacitinib citrate serving as the posi-tive control.After 28 days of intervention with dif-ferent doses of Yiqi Huoluo Formula,the degree of joint swelling and the arthritis index scores of the rats were observed.Pathological and morphologi-cal changes in the joints were evaluated using HE staining.The serum levels of IL-17,IL-6,TNF-α,and IL-10 were detected by ELISA.The proportions of Th17 and Treg cells in peripheral blood were mea-sured by flow cytometry.The mRNA expression lev-els of RORγt and Foxp3 in synovial tissues were de-tected by qRT-PCR.The protein expression levels of RORγt,Foxp3,p-JAK2,and p-STAT3 in synovial tis-sues were determined by Western blot.RESULTS:Yiqi Huoluo Formula effectively reduced the degree of joint swelling and the arthritis index scores in CIA rats(P<0.01),and improved the histopathological damage of the joint tissues.It decreased the serum levels of IL-17,IL-6,and TNF-α,as well as the Th17/Treg ratio in peripheral blood,while increas-ing the IL-10 levels(P<0.05,P<0.01).The formula down-regulated the mRNA and protein expression levels of RORγt and up-regulated the mRNA and protein expression levels of Foxp3 in the synovial tissues of rats(P<0.05,P<0.01).In addition,it signif-icantly inhibited the protein expression levels of p-JAK2 and p-STAT3 in synovial tissues(P<0.05,P<0.01).CONCLUSION:Yiqi Huoluo Formula can sig-nificantly alleviate arthritis symptoms in CIA model rats by inhibiting the JAK2/STAT3 signaling pathway and thereby regulating the Th17/Treg balance.关键词
益气活络方/类风湿关节炎/JAK2/STAT3通路/Th17/Treg 平衡Key words
Yiqi Huoluo Formula/rheumatoid arthritis/JAK2/STAT3 pathway/Th17/Treg balance分类
医药卫生引用本文复制引用
张浩,王海东,李浩林,陈平,王爱华,程伟刚,柏倩,杨娟娟,杨玉萍,张智敏..益气活络方通过调控JAK2/STAT3通路对类风湿关节炎模型大鼠Th17/Treg平衡的影响[J].中国临床药理学与治疗学,2026,31(5):577-584,8.基金项目
国家中医药管理局高水平重点学科建设项目(国中医药人教函[2022]226号) （国中医药人教函[2022]226号）
国家自然科学基金项目(82260880) （82260880）
甘肃省中医药科研项目(GZKG-2025-65) （GZKG-2025-65）
