中国临床药理学与治疗学2026，Vol.31Issue(5)：585-595,11.DOI:10.12092/j.issn.1009-2501.2026.05.002
法半夏通过巨噬细胞和成纤维细胞的Crosstalk减轻COPD损伤机制研究
Mechanism study of Processed Pinellia ternata alleviating COPD in-jury through macrophages and fibroblasts Crosstalk
摘要
Abstract
AIM:To explore the mechanism of Processed Pinellia ternata in treating chronic ob-structive pulmonary disease(COPD).METHODS:Forty male C57BL/6J mice were randomly divided into control group(n=8),model group(n=12),Pro-cessed Pinellia ternata group(1.5 g/kg,n=12),and aminophylline group(1 mg/kg,n=8).COPD model was established using smoke inhalation combined with lipopolysaccharide nasal drops.HE staining was employed to observe pathological damage in lung tissue.Lung tissue fibrosis was detected by Masson,MMP2,and MMP9 staining.Fibroblast transformation was tested α-SMA staining.CD206 and F4/80 staining were used to evaluated M2 macrophages polarization.The expression of TGF-β1 and Arg-1 genes were checked though qPCR,the level of Stat1 and p-Stat1 proteins were measured by Western blotting.In addition,macrophages and fibroblasts were co-cultured by transwell chambers,the(cells)were divided into blank control group,normal control group,model group,and low-,medi-um-,high-dose Pinellia ternata group groups(10,20,40 μg/mL,respectively),the fibroblasts transfor-mation was detected by α-SMA staining.RESULTS:Processed Pinellia ternata can significantly alleviate COPD injury(P<0.01),inhibit lung tissue prolifera-tion and fibrosis(P<0.001),reduce the relative ex-pression levels of TGF-β1 and Arg-1 genes(P<0.05),inhibit fibroblast transformation(P<0.05),reduce M2 macrophage polarization(P<0.05)and increase the p-Stat1/Stat1 ratio(P<0.05).CONCLUSION:Pro-cessed Pinellia ternata improves the fibrosis pro-cess in COPD by macrophages and fibroblasts crosstalk blocking,which may be related to sup-pressed M2 macrophage polarization and activated the Stat1 pathway.关键词
法半夏/慢性阻塞性肺疾病/纤维化/CrosstalkKey words
Processed Pinellia ternata/chronic obstructive pulmonary disease(COPD)/fibrosis/Crosstalk分类
医药卫生引用本文复制引用
魏宗烽,谭金龙,孙梦涵,朱乃亮,翟莹莹,陈琼,毕晶晶,梁利香,宋敏..法半夏通过巨噬细胞和成纤维细胞的Crosstalk减轻COPD损伤机制研究[J].中国临床药理学与治疗学,2026,31(5):585-595,11.基金项目
国家自然科学基金青年项目(21702051) （21702051）
河南省自然科学基金面上项目(232300420065) （232300420065）
河南省重点研发与推广专项(232102310364 （）
252102311246) （）
河南省重大专项(241111311400) （241111311400）
河南省高校重点科研项目(24B3600014) （24B3600014）
信阳农林学院青年基金项目(QN2023025 （）
QN2023028) （）
