中国美容医学2026，Vol.35Issue(6)：1-6,6.
皮肤镜结合皮肤生理指标检测在3%十二烷基硫酸钠斑贴试验刺激反应中的应用
Application of Dermoscopy and Skin Physiological Index to the 3％ Sodium Dodecyl Sulfate in Patch Test Irritation Response
摘要
Abstract
Objective To explore the application value of the result evaluation method combining dermoscopy and skin physiological index measurement in shortening the patch test cycle,improving the accuracy of patch test result judgment,and increasing the detection rate of subjects or patients.Methods Skin irritation response models were formed by applying 3%sodium dodecyl sulfate patches to 14 healthy subjects for 6 hours and 24 hours respectively.Meanwhile,distilled water and blank filter paper were used as negative and blank controls at 0.5 hours,24 hours and 48 hours after removing the patches.Visual scoring was conducted respectively,and non-invasive skin physiological indicators such as skin water content,TEWL,and skin erythema index a* value were detected.Meanwhile,dermoscopy was used to observe the characteristics under dermoscopy.Results In the 6 h group,there were 5 grade Ⅳ reactions,6 grade Ⅱ reactions,and 3 grade Ⅰ reactions in the 3%SLS-positive sites.In the 24 h group,there were 14 grade Ⅳ reactions in the 3%SLS-positive sites.In the 6 h and 24 h groups,skin water content at the 3%SLS-positive control site was significantly higher than that at the negative and blank control sites at 0.5 hours after spot tester removal(P＜0.05),and skin water content at the 3%SLS-positive control site was significantly lower than that at the negative control site at 48 hours after spot tester removal(P＜0.05).In the 6 h and 24 h groups,TEWL and erythema index a* were significantly higher at the 3%SLS positive control site than at the negative control site and blank control site at 0.5,24,and 48 hours after removal of the spot tester(P＜0.05).In the 6 h group,dermoscopy of the 3%SLS-positive control site showed irritant reaction features such as patchy mild erythema and scattered linear vasodilation on an orange background.In the 24 h group,dermoscopy of the 3%SLS-positive control site showed features such as more pronounced erythema,more linear vasodilation,and brownish-black scaling on a pink background.Conclusion Application of 3%SLS for 6 hours resulted in a positive skin patch test irritation model consistent with the effect of 24 hours of application.If the sensitizing substance is a potent sensitizer,the application time of the human skin patch test can be shortened to 6 hours,which may both reduce the total patch test time and reduce the adverse reactions caused by the potent sensitizer.关键词
皮肤斑贴试验/刺激反应/十二烷基磺酸钠/皮肤镜/皮肤角质层含水量/经皮水分丢失量(TEWL)/红斑指数Key words
skin patch test/irritation response/sodium dodecyl sulfate/dermoscopy/cuticle water content/transdermal water loss(TEWL)/erythema index分类
医药卫生引用本文复制引用
叶聪秀,易金玲,苏真,林燕雪,赖维..皮肤镜结合皮肤生理指标检测在3%十二烷基硫酸钠斑贴试验刺激反应中的应用[J].中国美容医学,2026,35(6):1-6,6.基金项目
国家药品监督管理局化妆品风险评估重点实验室开放性课题(编号:KF2022009) （编号:KF2022009）
