中国人兽共患病学报2026，Vol.42Issue(5)：449-456,8.DOI:10.3969/j.issn.1002-2694.2026.00.066
"One Health"监测中IncP6型质粒基因组分析与全球质粒序列比较
IncP6 plasmid genomic analysis in"One Health"surveillance and comparison of global plasmid sequences
摘要
Abstract
This study was aimed at exploring the global dissemination and genomic characteristics of IncP6-type plasmids carry-ing the Klebsiella pneumoniae carbapenemase(KPC)-encoding blaKPC-2 from a One Health perspective.We analyzed global distribu-tion features of IncP6 plasmids by using the Plasmids in Pathogens Database(PIPdb).Human-,animal-,environmental-,and food-derived samples were collected from Henan Province,China,under the One Health framework.We subsequently screened for carbapenem-resistant strains,performed plasmid incompatibility group typing on the resistant strains,and conducted preliminary identification and antimicrobial susceptibility testing on the screened carbapenem-resistant strains bearing IncP6-type plasmids.Se-quences of chromosomal and plasmid genomes were obtained through combined second-and third-generation sequencing technolo-gies.The genetic correlation between the IncP6 plasmid sequences carrying the blaKPC-2 gene and similar plasmid sequences in global public databases was analyzed.IncP6 plasmids demonstrated pandemic spread;were carried by bacteria from 39 species across 17 gen-era;and were detected in animal,human,food,and environmental samples.The blaKPC-2-carrying IncP6 plasmids exhibited broad host adaptability,extensive geographical distribution,and cross-niche persistence.An environmental isolate of Raoultella ornithino-lytica from Henan carried an IncP6 plasmid bearing blaKPC-2.This strain displayed a multidrug-resistant phenotype with multiple resis-tance genes.The identified IncP6 plasmid showed a certain genetic distance from related plasmids in public databases,and carried both the epidemic core resistance structure(ISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27)and a novel mobile genetic element(IS15DIV/msrE/mphE/IS15DIV).The IncP6 plasmid is widely present globally.The blaKPC-2-carrying IncP6 plasmid demonstrated stability and trans-missibility,and has been transferred across animal-human-environmental compartments.Future monitoring of the molecular charac-teristics and global spread of the blaKPC-2-carrying IncP6 plasmid from a One Health perspective is therefore important.关键词
同一健康/KPC-2/IncP6型质粒/解鸟氨酸拉乌尔菌/致病菌质粒数据库PIPdbKey words
One Health/KPC-2/IncP6 plasmid/Raoultella ornithinolytica/plasmids in pathogens database分类
医药卫生引用本文复制引用
魏薇,李臻鹏,刘子瑜,彭遥,赵嘉咏,卢昕.."One Health"监测中IncP6型质粒基因组分析与全球质粒序列比较[J].中国人兽共患病学报,2026,42(5):449-456,8.基金项目
国家重点研发项目(No.2024YFC3406300,No.2022YFC2303900) National Key Research and Development Program of China(No.2024YFC3406300,No.2022YFC2303900) （No.2024YFC3406300,No.2022YFC2303900）
