中国人兽共患病学报2026，Vol.42Issue(5)：490-495,6.DOI:10.3969/j.issn.1002-2694.2026.00.071
弓形虫章鱼碱脱氢酶Tg ODH多克隆抗体的制备与初步应用
Preparation and preliminary application of polyclonal antibodies against the octopine dehydrogenase protein of Toxoplasma gondii
摘要
Abstract
Toxoplasma gondii,an important zoonotic parasitic protozoan belonging to the apicomplexans,exhibits remarkable plas-ticity in its central carbon metabolism,thereby enabling its adaptation to diverse parasitic environments.Although several studies have elucidated the important roles of metabolic enzymes in Toxoplasma proliferation and dissemination,the functions of a subset of these enzymes remain uncharacterized.Herein,we focused on the T.gondii octopine dehydrogenase protein(ODH).Bioinformatic analysis revealed that ODH is a stable hydrophilic protein lacking signal peptides and transmembrane domains,and containing two conserved functional domains:an octopine dehydrogenase domain(208-378 aa)and a prephenate dehydrogenase domain(78-123 aa).We gen-erated polyclonal antibodies in mice immunized with a specific short peptide.Western blotting detected a specific band around 50 kDa,which was consistent with the predicted molecular weight of ODH.Indirect immunofluorescence assays further demonstrated the pro-tein's localization predominantly to the parasite cytoplasm.Together,these results indicated the successful production of mouse-derived polyclonal antibodies against ODH and delineated its subcellular localization,thereby providing a foundation for further investi-gation of the biological roles of ODH in T.gondii.关键词
弓形虫/多克隆抗体/ODH蛋白/免疫荧光Key words
Toxoplasma gondii/polyclonal antibodies/ODH protein/immunofluorescence分类
医药卫生引用本文复制引用
曹贝,李俊杰,徐凯,佘臣恩,朱艳,邹依琪,杨旭柯..弓形虫章鱼碱脱氢酶Tg ODH多克隆抗体的制备与初步应用[J].中国人兽共患病学报,2026,42(5):490-495,6.基金项目
安徽省高等学校科学研究重点项目(No.2023AH050686) （No.2023AH050686）
安徽医科大学"早期接触科研"训练计划项目(No.2024-ZQKY-016) Key Research and Development Project of Higher Education Institutions in Anhui Province(No.2023AH050686) （No.2024-ZQKY-016）
the Early Scientific Research Exposure Training Program of Anhui Medical University(No.2024-ZQKY-016). （No.2024-ZQKY-016）
