中国人兽共患病学报2026，Vol.42Issue(5)：502-509,8.DOI:10.3969/j.issn.1002-2694.2026.00.074
ROS/COX-2调控的线粒体自噬在BCG感染巨噬细胞凋亡中的作用及机制研究
Study on the role and mechanism of ROS/COX-2-regulated mitophagy in BCG-infected macrophage apoptosis
摘要
Abstract
This study investigated the regulatory role of reactive oxygen species(ROS)/cyclooxygenase-2(COX-2)-mediated mitophagy in Bacillus Calmette-Guérin(BCG)-induced apoptosis in RAW264.7 cells.Direct immunofluorescence double staining was used to observe mitophagy levels after BCG infection,and the protein expression of PTEN-induced putative kinase 1(PINK1),RBR E3 Ubiquitin Protein Ligase(Parkin),Microtubule-associated protein light chain 3(LC3),Translocase of the outer mitochon-drial membrane 20(TOM20),and Sequestosome 1(p62)was assessed via western blotting.To examine the involvement of the PINK1/Parkin pathway,PINK1 we transfected siRNA into the cells and investigated COX-2 regulation with COX-2 siRNA.The ef-fects of reactive oxygen species(ROS)were analyzed by treatment of the cells with 5 mmol/L N-acetylcysteine(NAC).BCG infection significantly increased the colocalization of LC3 with mitochondria,while upregulating the expression of PINK1,Parkin,and LC3 II,and downregulating TOM20 and p62.The PINK1 siRNA intervention decreased BCG-induced colocalization of LC3 and TOM20;de-creased PINK1,Parkin,and LC3 II expression;and increased TOM20 expression.BCG infection also enhanced COX-2 mRNA and protein expression.Inhibition of COX-2 impaired BCG-induced activation of the PINK1/Parkin pathway and further increased apopto-sis.Treatment with NAC suppressed BCG-induced ROS elevation and activation of the COX-2/PINK1/Parkin signaling pathway.Our findings suggest that ROS/COX-2-mediated mitophagy activation plays a crucial role in attenuating BCG-induced apoptosis in RAW264.7 cells.关键词
线粒体自噬/结核病/卡介苗/细胞凋亡/COX-2Key words
mitophagy/tuberculosis/Bacillus Calmette-Guerin/apoptosis/COX-2分类
医药卫生引用本文复制引用
陈飞飞,郑永智,吴素芳,康乾,晋春阳..ROS/COX-2调控的线粒体自噬在BCG感染巨噬细胞凋亡中的作用及机制研究[J].中国人兽共患病学报,2026,42(5):502-509,8.基金项目
河南省高等学校重点科研项目计划(No.23A360023) Key Scientific Research Project Plan of Universities in Henan Province(No.23A360023) （No.23A360023）
