中国实验动物学报2026，Vol.34Issue(5)：655-664,10.DOI:10.3969/j.issn.1005-4847.2026.05.003
基于"以方测证"的寒湿泄泻大鼠模型建立与验证
Establishment and verification of a rat model of cold-dampness diarrhea based on"diagnosis syndrome by prescription"
摘要
Abstract
Objective This study is based on the"diagnosis syndrome by prescription"approach and adopts a combination of internal and external factors to construct a rat model of cold-damp diarrhea.Methods 48 rats were randomly divided into a blank control(CON,n=12)group,a cold-damp diarrhea model(CDS,n=12)group,a Lizhong decoction intervention(LCD,n=12)group,and a Pulsatilla decoction intervention(PCD,n=12)group.The model construction used a composite factor:internal factors(psyllium seed combined with ice water gavage)and external factors(cold-damp environmental stimulation)were combined to replicate the rat model of cold-damp diarrhea.The Lizhong decoction and Pulsatilla decoction interventions were carried out for the analysis of the diagnosis syndrome by prescription,and the clinical manifestations of each group of rats were recorded.The atpase activity and the levels of serum interleukin(IL)-6,IL-10,tumor necrosis factors(TNF)-α,and IL-1β were measured in all groups.Results Compared with normal rats,rats in the model group exhibited increased rates of loose stools,higher fecal water content,and elevated diarrhea indices,consistent with the diagnostic criteria for primary and secondary symptoms associated with cold-dampness diarrhea.Notably,serum Ca2+-Mg2+ATPase and Na+-K+ATPase activities were significantly decreased in rats in the model group,while IL-6,IL-1β,and TNF-αlevels were markedly increased and IL-10 levels declined.Prescription testing indicated that Lizhong Decoction had a significant impact on these parameters among rats suffering from cold-dampness diarrhea,while Pulsatilla decoction had no notable effects.Conclusion An effective animal model for cold-dampness diarrhea was successfully established by a"combination of internal causes+external causes",providing a robust methodological foundation for further investigations into the mechanisms underlying diarrhea-related diseases.关键词
寒湿泄泻/动物模型/理中汤/白头翁汤/以方测证Key words
cold-damp diarrhea/animal model/Lizhong decoction/Pulsatilla decoction/diagnosis syndrome by prescription分类
生物科学引用本文复制引用
史花蓉,张昊,米文文,纪鹏,魏彦明,华永丽..基于"以方测证"的寒湿泄泻大鼠模型建立与验证[J].中国实验动物学报,2026,34(5):655-664,10.基金项目
国家自然科学基金项目(32260898),甘肃省大学生创新创业训练计划项目(S202410733019),国家肉牛/牦牛产业技术体系(CARS-37),甘肃省科技计划(24YFNA016),甘肃省现代寒旱特色农业科技支撑项目(KJZC-2025-14),甘肃农业大学"伏羲杰出人才培育计划"(Gaufx-03J01).Funded by National Natural Science Foundation of China(32260898),the Gansu Province College Students' Innovation and Entrepreneurship Training Program Project(S202410733019),China Agriculture Research System of MOF and MARA(CARS-37),Gansu Province Science and Technology Plan(24YFNA016),Modern Cold and Drought Characteristic Agricultural Science and Technology Support Project of Gansu Province(KJZC-2025-14),Gansu Agricultural University's"Fuxi Outstanding Talent Cultivation Program"(Gaufx-03J01). （32260898）
