中国实验动物学报2026，Vol.34Issue(5)：665-675,11.DOI:10.3969/j.issn.1005-4847.2026.05.004
登革病毒2型感染对新型hTim4基因修饰小鼠脾的病理损伤
A novel hTim4 genetically modified mouse model reveals enhanced splenic pathogenesis upon dengue virus serotype 2 challenge
摘要
Abstract
Objective To investigate pathological changes in the spleens of human T cell immunoglobulin and mucin domain containing 4(hTim4)genetically modified mice(hTim4 mice)induced by serotype 2 dengue virus Tr.1751(DENV2).Methods The hTim4 mice were intracerebrally inoculated with DENV2(1× 103 PFU/mL).After inoculation,body mass was monitored,and deaths were documented.Viremia was recorded,along with viral load and pathological changes in the spleens of dying mice;vascular leakage was also determined.Results Mice inoculated with DENV2 exhibited continuous body mass reduction,all succumbing to infection.Significant vascular leakage in spleens,lungs,livers,and intestines was observed,along with severe spleen atrophy.Spleen white and red pulp boundaries became blurry,and cell counts decreased in both PALS and margin zones;apoptotic cells were scattered in white pulp,especially in the margins.Conclusions DENV2 infection caused splenic hemorrhage,cell reduction,and atrophy in hTim4 mice.关键词
登革热/登革病毒2型/hTim4转基因小鼠/脾萎缩/脾出血/病理Key words
dengue hemorrhagic fever/dengue virus serotype 2/hTim4 transgenic mice/spleen atrophy/splenic hemorrhage/pathology分类
生物科学引用本文复制引用
杨远松,翟世杰,范昌发,王依宁,王誉雅,刘甦苏,吴勇,凌晨,柯璐,熊芮,赵皓阳..登革病毒2型感染对新型hTim4基因修饰小鼠脾的病理损伤[J].中国实验动物学报,2026,34(5):665-675,11.基金项目
国家重点研发计划(2023YFC2307905).Funded by the National Key R & D Program of China(2023YFC2307905). （2023YFC2307905）
