中国实验动物学报2026，Vol.34Issue(5)：685-699,15.DOI:10.3969/j.issn.1005-4847.2026.05.006
类风湿关节炎SD大鼠模型的对比研究
Comparative investigation of a rheumatoid arthritis model in SD rats
摘要
Abstract
Objective This study aimed to investigate the effects of different immunization sites(subcutaneous tail base versus subcutaneous plantar hind paw)and solvents(0.9％sodium chloride solution and 3.6％acetic acid)on model stability,inflammatory response,and pathological progression in SD rat models of collagen-induced arthritis(CIA)and adjuvant-induced arthritis(AIA),and to optimize the methodology for constructing the CIA rat model.Methods Methods One hundred and twenty female SD rats were randomly divided into 10 groups,including a blank group,an AIA group,and eight groups subjected to different CIA modeling protocols(distinguishing immunization site and solvent).Dynamic observation of arthritis index(AI),serum anti-cyclic citrullinated peptide(anti-CCP)and rheumatoid factor(RF)antibody levels,histopathological changes of the ankle joint,and protein and mRNA expression of IL-6 and IL-17 were carried out on days 7,14,21,28,and 35 after sensitization.Results The HAc-Hind Paw-Hind Paw group(3.6％acetic acid solvent+double subcutaneous plantar hind paw immunization)showed significantly higher arthritis index(AI)scores,serum anti-CCP and RF antibody levels,and local joint IL-6 and IL-17 expression compared with other CIA groups(P＜0.01);these indicators remained at a high and stable level throughout the entire experimental period.Hematoxylin-eosin(HE)staining of the ankle joint revealed a typical chronic and progressive pathological process,and this group was significantly superior to the traditional AIA model in mimicking human RA.Although the traditional AIA model elicited a strong early response,it showed significant pathological remission by day 35,exhibiting a self-limiting nature.The NS-Hind Paw-Hind Paw group induced persistent pathological changes,but the intensity of inflammation declined in the later stages.The HAc-Tail-Hind Paw group generated a strong systemic immune response,but the local joint pathology fluctuated greatly and exhibited poor stability.Conclusion The animal model established by the HAc-Hind-Paw-Hind Paw method exhibited pronounced and stable chronic,progressive rheumatoid arthritis symptoms and pathological features compared with the traditional AIA model and the HAc-Tail-Hind Paw model,accompanied by high levels of IL-6,IL-17,anti-CCP antibodies,and RF antibodies,and may represent an improved SD rat model of RA.关键词
类风湿关节炎/SD大鼠/胶原诱导性关节炎/佐剂诱导性关节炎Key words
rheumatoid arthritis/SD rat/collagen-induced arthritis/adjuvant-induced arthritis分类
生物科学引用本文复制引用
吴华华,陈跃平,黄川洪..类风湿关节炎SD大鼠模型的对比研究[J].中国实验动物学报,2026,34(5):685-699,15.基金项目
2025年中央财政医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)-中西医"旗舰"科室-骨伤科(桂财社函(2025)36号),广西自然科学基金(2024GXNSFAA010321),广西中医药大学A类"桂派中医药传承创新团队"(2022A004),广西中医药大学研究生教育创新计划项目(YCBXJ2025029,YCSY2025060).Funded by 2025 Central Government Subsidy for Enhancing Medical Service and Guarantee Capacity(Part of Inheritance and Development of Traditional Chinese Medicine)-Chinese and Western Medicine"Flagship"Department-Orthopedics and Traumatology Department(Guicai Shehan[2025]No.36),Guangxi Natural Science Foundation(2024GXNSFAA010321),Guangxi University of Chinese Medicine Category A"Guipai Chinese Medicine Inheritance and Innovation Team"(2022A004),Innovation Project of Guangxi Graduate Education of GXUCM(YCBXJ2025029,YCSY2025060). （中医药事业传承与发展部分）
