中国实验动物学报2026，Vol.34Issue(5)：728-739,12.DOI:10.3969/j.issn.1005-4847.2026.05.010
肺炎支原体R414北京流行株感染小鼠模型的研究
Study on a murine model of infection with the Mycoplasma pneumoniae R414 Beijing epidemic strain
摘要
Abstract
Objective To establish a murine Mycoplasma pneumoniae(Mp)model by intranasally infecting BALB/c mice with the reference strain FH and the Beijing epidemic strain R414,and to evaluate the similarities and differences in pathogenicity and immunopathology between R414 and the reference strain FH.Methods 3-week-old and 8-week-old BALB/c mice were intranasally infected with FH or R414 strains.Control groups received intranasal 0.9％sodium chloride solution.Pathogen load,pathological changes,and transcriptome alterations in lung tissues,as well as peripheral blood immune cell and cytokine levels,were measured on days 6,9,and 12 post-infection.Results Mice infected with Mycoplasma pneumoniae exhibited features of acute pneumonia(high pathogen load and significant inflammation on day 6,subsiding by days 9～12).No significant differences were observed between FH and R414 strains in pathogen load.Following FH infection,cellular immunity in 8-week-old mice was activated earlier than in 3-week-old mice;moreover,the number of differentially expressed genes in the transcriptome of 8-week-old mice significantly exceeded that of 3-week-old mice.6 d after R414 infection in 3-week-old mice,peripheral blood IP-10 levels increased.6 d after R414 infection in 8-week-old mice,the proportion of monocytes increased,and TNF-αlevels rose.The R414 strain induced a"viral response"pathway in 3-week-old mice,while in 8-week-old mice,it primarily activated pathways such as the"IL-17 signaling pathway"and"neutrophil chemotaxis".Conclusions The R414 strain induced differentiated host immune responses compared to the reference strain.These differences were correlated with the age of the mice and warrant further investigation.关键词
肺炎支原体/动物模型/细胞因子/转录组学Key words
Mycoplasma pneumoniae/animal model/cytokines/transcriptomics分类
生物科学引用本文复制引用
肖好好,薛冠华,郭智,李夏,袁静,向志光..肺炎支原体R414北京流行株感染小鼠模型的研究[J].中国实验动物学报,2026,34(5):728-739,12.基金项目
中国医学科学院医学与健康科技创新工程(2021-I2M-1-039),国家自然科学基金(82241056),中国医学科学院中央级公益性科研院所基本科研业务费项目(2023-PT180-01),北京市自然科学基金(7242015).Funded by CAMS Innovation Fund for Medical Sciences(2021-I2M-1-039),National Natural Science Foundation of China(82241056),the Non-Profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2023-PT180-01),Beijing Natural Science Foundation(7242015). （2021-I2M-1-039）
