中国比较医学杂志2026，Vol.36Issue(8)：61-70,10.DOI:10.3969/j.issn.1671-7856.2026.08.006
LncRNA UCA1靶向miR-520a-5p对膀胱癌细胞增殖、凋亡及放射敏感性的影响
Effects of LncRNA UCA1 on proliferation,apoptosis and radiosensitivity of bladder cancer cells by targeting miR-520a-5p
摘要
Abstract
Objective To investigate the effects of urothelial carcinoma antigen 1 long non-coding RNA(LncRNA UCA1)on the proliferation,apoptosis,and radiosensitivity of bladder cancer cells by regulating miR-520a-5p.Methods LncRNA UCA1 and miR-520a-5p were detected in normal bladder epithelial(HCV-29)and human bladder cancer(SW780,HT1376,BIU87 and T24)cell lines by quantitative reverse transcription-polymerase chain reaction.T24 cells were treated with different radiation doses(0～8 Gy)and cell proliferation activity was detected.T24 cells were assigned to Control,sh-NC,sh-UCA1,sh-UCA1+anti-miR-NC,and sh-UCA1+anti-miR-520a-5p groups.Cells in each group were treated with a radiation dose of 2 Gy,and cell proliferation,apoptosis,and cyclin D1,Ki-67,Bax,and Caspase-3 protein expression levels were detected.The targeting relationship between LncRNA UCA1 and miR-520a-5p was verified by dual-luciferase reporter gene,fluorescence in situ hybridization,and RNA immunoprecipitation assays.A nude mouse tumor xenotransplantation model was established,and the effect of LncRNA UCA1 on the radiosensitivity of bladder cancer was verified.Results LncRNA UCA1 was increased and miR-520a-5p was decreased in bladder cancer cell lines.Treating T24 cells with different radiation doses(0～8 Gy)reduced cell proliferation activity in the sh-UCA1 group compared with the sh-NC group,in line with increasing radiation dose(P＜0.05).T24 cells and a radiation dose of 2 Gy were therefore selected for subsequent experiments.Knockdown of LncRNA UCA1 in T24 cells inhibited cell proliferation,promoted cell apoptosis,increased radiosensitivity,down-regulated cyclin D1 and Ki-67,and upregulated Bax and Caspase-3(P＜0.05).miR-520a-5p antagonist weakened the impacts of LncRNA UCA1 knockdown on the proliferation,apoptosis,and radiosensitivity of bladder cancer cells(P＜0.05).LncRNA UCA1 targeted the negative regulation of miR-520a-5p.Knockdown of LncRNA UCA1 combined with radiotherapy inhibited the growth of bladder cancer tumor transplants and increased their radiosensitivity(P＜0.05).Conclusions LncRNA UCA1 is up-regulated in bladder cancer cells.Knockdown of LncRNA UCA1 can inhibit cell proliferation,induce apoptosis,and enhance the radiosensitivity of bladder cancer cells by up-regulating miR-520a-5p.关键词
LncRNA UCA1/miR-520a-5p/膀胱癌/放疗敏感性/增殖/凋亡Key words
LncRNA UCA1/miR-520a-5p/bladder cancer/radiosensitivity/proliferation/apoptosis分类
医药卫生引用本文复制引用
余洋,熊飞,陈晓波,孙伟,张炜..LncRNA UCA1靶向miR-520a-5p对膀胱癌细胞增殖、凋亡及放射敏感性的影响[J].中国比较医学杂志,2026,36(8):61-70,10.基金项目
武汉市医学科研项目(WX21B04). （WX21B04）
