中国兽医科学2026，Vol.56Issue(5)：578-586,9.DOI:10.16656/j.issn.1673-4696.2026.0096
表达O型口蹄疫病毒中和抗体转基因细胞系的构建与应用
Development and application of transgenic cell lines expressing neutralizing antibodies against foot-and-mouth disease virus serotype O
摘要
Abstract
This study aimed to investigate whether cell lines expressing foot-and-mouth disease virus(FMDV)neutralizing antibodies(NAbs)could inhibit viral infection and replication.First,plasmids expressing FMDV NAbs in the form of single-chain fragments variable(scFv)were constructed.To further enhance the secretion efficiency of NAbs into the extracellular space,four secretory signal peptides were added into each scFv.The expression vectors were transfected into BHK-21 cells via liposome-mediated transfect ion,fol lowed by puromycin selection to obtain the corresponding transgenic cell lines.Subsequently,FMDV replication in the transgenic cell lines was assessed using Western-blot and TCID50 assays.Results demonstrated that the mIg κ-signal peptide-tagged NAbs significantly inhibited FMDV replication in BHK-21 cells,and the degree of cytopathic effect induced by viral infection was markedly reduced.These findings indicate that scFv-formulated NAbs can potently suppress FMDV replication in vitro,offering novel insights for controlling livestock epidemics.关键词
口蹄疫病毒/中和抗体/单链抗体/转基因细胞系Key words
foot-and-mouth disease virus/neutralizing antibody/single-chain antibody/transgenic cell lines分类
农业科技引用本文复制引用
杨彪,冯涛,包世俊,郑海学,齐晓兰,吴国华,晏子龙,郝荣增,郑东东,谢正杰,刘宝,闫祯芳..表达O型口蹄疫病毒中和抗体转基因细胞系的构建与应用[J].中国兽医科学,2026,56(5):578-586,9.基金项目
甘肃省青年科技基金项目(25JRRA444) （25JRRA444）
"十四五"广东省揭榜挂帅项目(2024KJ14) （2024KJ14）
甘肃省科技重大专项计划项目(24ZD13NA008) （24ZD13NA008）
兰州市科技计划项目(2024-8-12) （2024-8-12）
