中国兽医科学2026，Vol.56Issue(5)：610-616,7.DOI:10.16656/j.issn.1673-4696.2026.0075
马链球菌马亚种MDMC-0316株主要毒力蛋白Eq5和IdeE2的免疫原性研究
Immunogenicity of Eq5 and IdeE2 virulence proteins of Streptococcus equi subsp.equi strain MDMC-0316
摘要
Abstract
This article was aimed to explore the potential of Eq5 and IdeE2 proteins from Streptococcus equi subsp.equi(SEE)strain MDMC-0316 as subunit vaccine targets,the eq5 and ideE2 genes were amplified by PCR,sequenced,fol lowed by immunoinformatic analysis of their corresponding amino acid sequences.The genes were cloned into the pET-28a vector for expression and purification.Purified proteins were used to immunize mice,and antibody titers along with cytokine levels were measured by ELISA.Simulated analysis results showed that both Eq5 and IdeE2 proteins exhibited good immunogenicity and strong stimula-tory effects on IL-2 and IFN-γ production.Immunization of mice with either protein induced high levels of IgG,and the D450nm values of the immune serum antibodies against Eq5 and IdeE2 were both greater than 1.0 at a titer of 1∶51 200.In the Eq5-immunized group,the concentrations of IL-2,IL-4,and IFN-γ were 61.8 pg/mL,105.8 pg/mL,and 151.3 pg/mL,respectively.In the IdeE2-immunized group,the levels were 89.1 pg/mL for IL-2,98.0 pg/mL for IL-4,and 173.5 pg/mL for IFN-γ.Compared with Eq5 protein,IdeE2 protein induced significantly higher production of IL-2 and IFN-γ,which is consistent with the predic-tions from the immunoinformatic analysis.This study provides candidate antigens for the development of a novel subunit vaccine against SEE.关键词
马链球菌马亚种/Eq5蛋白/IdeE2蛋白/免疫原性Key words
Streptococcus equi subspecies equi/Eq5/IdeE2/immunogenicity分类
农业科技引用本文复制引用
韩博栋,梁建忠,朝木丽格,欧云文,白文成,王雪,李旭阳,刘芳,格日勒图..马链球菌马亚种MDMC-0316株主要毒力蛋白Eq5和IdeE2的免疫原性研究[J].中国兽医科学,2026,56(5):610-616,7.基金项目
内蒙古自治区自然科学基金项目(2025MS03148) （2025MS03148）
鄂尔多斯市重点研发计划项目(YF20240011) （YF20240011）
国家自然科学基金项目(31360602) （31360602）
阿拉善盟科技计划项目(AMKJ2023-03) （AMKJ2023-03）
