中国兽医杂志2026，Vol.62Issue(6)：10-20,11.DOI:10.20157/j.cnki.zgsyzz.2026.06.002
J亚群禽白血病病毒分离株的分离鉴定和全基因组测序分析
Isolation,Identification and Whole Genome Sequencing Analysis of Arian Leukosis Virus Subtype J Isolates
摘要
Abstract
To investigate the infection status of avian leukosis(AL)in Hebei Province,suspected tumor-associated dead chickens were collected between 2023 and 2024 and screened for avian leukosis virus(ALV)by polymerase chain reaction(PCR).ALV-positive samples were inoculated into chicken embryo fibroblasts(CEF)for indirect immunofluorescence assay(IFA),viral subgroup identification,and chick infection experiments.The whole genome of the isolated strains was subsequently sequenced and analyzed.The results showed that 53 of the 63 samples were ALV-positive.Based on IFA and viral subgrouping,one ALV subgroup A(ALV-A)strain and fourteen ALV subgroup J(ALV-J)strains were obtained.A chick infection experiment identified one highly pathogenic ALV-J strain,designated HB.QHD.7.Genome sequencing and analysis revealed that the HB.QHD.7 isolate had a full-length genome of 7 535 bp,sharing 87.7%nucleotide identity with the UK prototype strain HPRS-103 and the highest identity(89.2%)with the Guangxi strain GX16ZS01.Phylogenetic analysis showed that HB.QHD.7 clustered within the same major branch as reference ALV-J strains,indicating a close evolutionary relationship.The envelope protein(env)gene of HB.QHD.7 showed the highest homology(89.2%)with the Yunnan strains YNJC2201 and YNXC2201.The surface glycoprotein 85(gp85)gene exhibited the highest homology(86.5%)with the Yunnan strains YNJC2201 and YNXC2202,and mutations were identified in variable region 1(vr1),hypervariable region 1(hr1),and hypervariable region 2(hr2).The 3 ′ long terminal repeat(3 ′-LTR)sequence showed the highest homology(69.1%)with the Guangxi strain GX14NN01.In addition,the 3′ untranslated region(3′UTR)lacked the rTM region,partially lacked the E element,and contained a continuous 10-nucleotide deletion in the U3 region.These findings indicate that ALV infection is a major cause of tumor diseases in breeder chickens in Hebei Province and that such infections are widespread.This study enriches the genetic and evolutionary data of ALV and provides valuable information for future research and the prevention and control of ALV in breeder flocks.关键词
J亚群禽白血病病毒(ALV-J)/全基因组测序(WGS)/病毒分离Key words
avian leukosis virus subtype J(ALV-J)/whole genome sequencing(WGS)/virus isolation分类
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伊雪燕,李佩国,周润雨,殷鹤予,顾宇华,匡华丽,贾青辉,张志强,吴同垒,李蕴玉..J亚群禽白血病病毒分离株的分离鉴定和全基因组测序分析[J].中国兽医杂志,2026,62(6):10-20,11.基金项目
河北省现代农业产业体系蛋禽创新团队资助项目(HBCT2018150206,HBCT2024260209) （HBCT2018150206,HBCT2024260209）
河北省现代农业产业体系肉禽创新团队资助项目(HBCT2024270207) （HBCT2024270207）
