中成药2026，Vol.48Issue(5)：1429-1435,7.DOI:10.3969/j.issn.1001-1528.2026.05.001
HPLC-MS/MS法同时测定参芎益心颗粒中34种成分的含量
Simultaneous content determination of thirty-four constituents in Shenxiong Yixin Granules by HPLC-MS/MS
摘要
Abstract
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of ginsenosides[ginsenosides Rg1,Re,Rf,Rb1,Ra1,Rc,Rg2,Rh1,(R)-Rg2,Rb2,(R)-Rh1,Rb3,Ro,Rd,Rg3,(R)-Rg3],phenolic acids(danshensu,protocatechuic acid,neochlorogenic acid,chlorogenic acid,protocatechuic aldehyde,cryptochlorogenic acid,caffeic acid,syringic acid,4-hydroxycinnamic acid,ferulic acid,rosmarinic acid,salvianolic acid B),tanshinones(dihydrotanshinone Ⅰ,tanshinone Ⅰ,cryptotanshinone,tanshinone Ⅱ A)and flavonoids(hyperoside,isoquercitrin)in Shenxiong Yixin Granules.METHODS The analysis was performed on a 30 ℃ thermostatic phenomenex Luna C18 coclumn(150 mm×4.6 mm,5 μm),with the mobile phase comprising of 0.01％formic acid-acetonitrile flowing at 0.4 mL/min,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Thirty-four constituents showed good linear relationships within their own ranges(r＞0.993 5),whose average recoveries were 89.33％-118.44％with the RSDs of 1.80％-8.39％.CONCLUSION This simple,feasible,stable and reliable method can be used for the quality control of Shenxiong Yixin Granules.关键词
参芎益心颗粒/化学成分/含量测定/HPLC-MS/MSKey words
Shenxiong Yixin Granules/chemical constituents/content determination/HPLC-MS/MS分类
医药卫生引用本文复制引用
陈秋谷,赖珍沁,吴圆圆,栾杰男,张尚斌,陈剑平..HPLC-MS/MS法同时测定参芎益心颗粒中34种成分的含量[J].中成药,2026,48(5):1429-1435,7.基金项目
深圳市科技创新委员会项目(JCYJ20220818103402006,ZDSYS201606081515458) （JCYJ20220818103402006,ZDSYS201606081515458）
深圳市医疗卫生"三名工程"项目(SZZYSM202111002) （SZZYSM202111002）
中医药广东省实验室项目(HQCML-C-2024005) （HQCML-C-2024005）
