中成药2026，Vol.48Issue(5)：1436-1443,8.DOI:10.3969/j.issn.1001-1528.2026.05.002
UPLC-MS/MS法同时测定青果丸中15种成分的含量
Simultaneous content determination of fifteen constituents in Qingguo Pills by UPLC-MS/MS
摘要
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of gallic acid,chlorogenic acid,cryptochlorogenic acid,luteoloside,isochlorogenic acid C,quercetin,daurisoline,harpagoside,luteolin,baicalein,methylophiopogonanone B,dauricine,paeoniflorin,baicalin and wogonin in Qingguo Pills.METHODS The analysis was performed on a 35 ℃ thermostatic Brownlee SPP C18 column(2.1 mm×100 mm,2.7 μm),with the mobile phase comprising of water(containing 0.1％formic acid and 5 mmol/L ammonium acetate)-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed.RESULTS Fifteen constituents showed good linear relationships within their own ranges(R2＞0.998 8),whose average recoveries were 94.52％-106.39％with the RSDs of 0.21％-3.94％.Twenty-two batches of samples were clustered into four categories,four principal components demonstrated the accumulative variance contribution rate of 83.808％,isochlorogenic acid C,luteoloside,gallic acid,chlorogenic acid,cryptochlorogenic acid,methylophiopogonanone B and daurisoline were taken as differential components.CONCLUSION This accurate and sensitive method can be used for the quality evaluation of Qingguo Pills.关键词
青果丸/化学成分/含量测定/UPLC-MS/MS/聚类分析/主成分分析/正交偏最小二乘判别分析Key words
Qingguo Pills/chemical constituents/content determination/UPLC-MS/MS/cluster analysis/principal component analysis/orthogonal partial least squares discriminant analysis分类
医药卫生引用本文复制引用
南丹慧,姚昱吏,康荣峰,马海棠,刘晓玲,李运,杨扶德..UPLC-MS/MS法同时测定青果丸中15种成分的含量[J].中成药,2026,48(5):1436-1443,8.基金项目
国家药品监督管理局中药材及饮片质量控制重点实验室项目(2024GSMPA-KL) （2024GSMPA-KL）
2021年度甘肃高等学校产业支撑计划项目(2021CYZC-40) （2021CYZC-40）
兰州市人才创新创业项目(2023-RC-9) （2023-RC-9）
