中成药2026，Vol.48Issue(5)：1457-1465,9.DOI:10.3969/j.issn.1001-1528.2026.05.005
鲜地黄在血热出血模型大鼠体内代谢产物鉴定
Identification of in vivo metabolites of fresh Rehmanniae Radix in blood heat and hemorrhage model rats
摘要
Abstract
AIM To identify the in vivo metabolites of fresh Rehmanniae Radix in blood heat and hemorrhage model rats.METHODS Sixteen rats were randomly divided into two groups and given intragastric administration of normal saline and fresh Rehmanniae Radix extract,respectively,after which ultra-high-performance liquid chromatography coupled with linear ion trap-tandem electrostatic field orbital high-resolution mass spectrometry(UHPLC-LTQ-Orbitrap-MS/MS)was adopted in the identification of prototypical components and metabolites in serum,urine and tissues(heart,liver,spleen,lung,kidney,small intestine).RESULTS Total 51 constituents were identified,including 6 prototypical components and 45 metabolites.In the serum,urine,heart,liver,spleen,lung,kidney,small intestine,5,6,1,1,1,1,1,1,1,4 prototypical components and 9,30,8,11,5,4,6,11 metabolites were identified,respectively.Fresh Rehmanniae Radix was metabolized by pathways such as dehydrogenation,reduction,dehydration,methylation and glucuronidation.CONCLUSION Fresh Rehmanniae Radix occurrs phase Ⅰ and phase Ⅱ metabolisms in blood heat and hemorrhage model rats in vivo,the metabolites are widely distributed in various main organs,which can provide basis for the research on pharmacodynamic material basis and action mechanisms of this medicinal material.关键词
鲜地黄/体内代谢产物/血热出血/组织分布/超高效液相色谱-线性离子阱串联静电场轨道高分辨质谱(UHPLC-LTQ-Orbitrap-MS/MS)分类
医药卫生引用本文复制引用
邱田田,王继舒,马美杰,薛淑娟,陈随清..鲜地黄在血热出血模型大鼠体内代谢产物鉴定[J].中成药,2026,48(5):1457-1465,9.基金项目
河南省高等学校重点科研项目计划(23A360002) （23A360002）
河南省科技攻关项目(242102310501,252102311279) （242102310501,252102311279）
