种子2026，Vol.45Issue(4)：15-25,11.DOI:10.16590/j.cnki.1001-4705.2026.04.015
基于miRNA测序的甘薯块根miRNA差异表达及其靶基因分析
Analysis of Differentially Expressed miRNAs and Their Target Genes in Ipomoea batatas Tuberous Roots via miRNA Sequencing
摘要
Abstract
MicroRNA(miRNA)is a type of endogenously encoded non-coding small RNA molecule,which plays a significant role in plant growth and development,hormone signal transduction and secondary metabolism regulation.In this study,using purple-fleshed Ipomoea batatas(PFSP)and white-fleshed Ipomoea batatas(WFSP)as materials,through small RNA high-throughput sequencing technology combined with bioinformatics analysis,the conserved and new miRNAs in the tubers of the 2 varieties at different anthocyanin accumulation periods were systematically identified,their potential target genes were predicted,and functional annotation was carried out.A total of 12 sRNA sequencing data samples were obtained.After quality control,the Clean Reads of each sample were over 10.69 M,and the Q30 value was above 96.80％.After alignment to the Ipomoea batatas reference genome(Ipomoea batatas.Version1),a total of 294 miRNAs were identified,including 9 known miRNAs and 285 newly predicted miRNAs.The length distribution showed that the most miRNAs were concentrated in 21 nt,and the first base at the 5'end preferred uracil(U).Further,using the TargetFinder software to predict the target genes of differentially expressed miRNAs and combined with GO and KEGG enrichment analysis,it was revealed that they were involved in biological processes such as plant hormone signal transduction,secondary metabolism and stress response.关键词
甘薯/microRNA/small RNA测序/靶基因/花青素/生物信息学分析Key words
Ipomoea batatas/microRNA/small RNA sequencing/target gene/anthocyanin/bioinformatics analysis分类
农业科技引用本文复制引用
熊军,唐秀桦,唐秀梅,韦民政..基于miRNA测序的甘薯块根miRNA差异表达及其靶基因分析[J].种子,2026,45(4):15-25,11.基金项目
广西重点研发计划项目(桂农科AB241484015) （桂农科AB241484015）
广西农业科学院基本科研业务专项(桂农科2026YT114、桂农科2022JM37) （桂农科2026YT114、桂农科2022JM37）
