种子2026，Vol.45Issue(4)：40-47,8.DOI:10.16590/j.cnki.1001-4705.2026.04.040
玉米赖氨酸代谢相关ZmL-PO基因的克隆及功能分析
Cloning and Function Analysis of the ZmL-PO Gene Related to Lysine Metabolism in Maize
摘要
Abstract
To explore the function of the ZmL-PO gene and to clarify the molecular regulatory mecha-nism of the increase in lysine(Lys)content in maize(Zea mays L.)kernels,using maize B73 as the material,the coding region of ZmL-PO was obtained through molecular cloning and its sequence was sequenced.Combined with bioinformatics analysis,the protein function of ZmL-PO was predicted,and the gene expression patterns in different tissues and at different stages of maize B73 were ana-lyzed.The results showed that the length of the CDS of the cloned ZmL-PO was 1 245 bp,and it was predicted to contain 415 amino acids with a molecular weight of approximately 43.94 kD and an isoe-lectric point of 5.79.Bioinformatics analysis indicated that ZmL-PO belonged to a hydrophilic protein located in the chloroplast,and its secondary structure was mainly α-helix.Systematic phylogenetic analysis showed that the evolutionary relationship between ZmL-POprotein and Sorghum bicolor was the closest.The qRT-PCR results showed that the expression level of ZmL-PO was the highest in the kernels 20 days after pollination.关键词
玉米/L-甲基哌啶氧化酶/生物信息学分析/功能分析Key words
Zea mays/L-pipecolate oxidase/bioinformatics analysis/function analysis分类
农业科技引用本文复制引用
邓磊,张开武,杨锦越,任洪,王伟..玉米赖氨酸代谢相关ZmL-PO基因的克隆及功能分析[J].种子,2026,45(4):40-47,8.基金项目
科研机构创新能力建设项目(黔科合服企[2022]007) （黔科合服企[2022]007）
贵州旱地粮食作物基因遗传转化平台建设(黔科中引地[2022]4011) （黔科中引地[2022]4011）
国家自然科学基金(32060461) （32060461）
国家玉米产业技术体系(CARS-02-91) （CARS-02-91）
