种子2026，Vol.45Issue(4)：48-55,8.DOI:10.16590/j.cnki.1001-4705.2026.04.048
大豆抗SCN基因rhg1与Rhg4变异位点及育种利用评价
Variation Sites and Breeding Utilization Evaluation of SCN Resistance Genes rhg1 and Rhg4 in Glycine max
摘要
Abstract
Using 50 Glycine max germplasm resources as materials,a genotype analysis of the main effective loci variation sites for resistance to cyst nematode(SCN)was conducted,and the resistance of the 3rd physio-logical race was identified,as well as the correlation analysis of phenotypic traits and principal component analysis,etc.The results showed that in the evaluation of resistance to SCN3,5 disease-resistant germplasms were selected(accounting for 10％).13 moderately resistant germplasms(accounting for 26％).Through the rhg1-570 marker,it was found that the detection rates of SNP_462A and SNP_774A alleles at the rhg1 locus were 72.22％and 61.11％respectively;through the Rhg4-SHMT marker,the detection rates of SNP_389G and SNP_510C alleles at the Rhg4 locus were 33.33％and 50.00％respectively.In the principal component analysis of the 13 disease-resistant germplasms,the cumulative contribution rate of the 4 principal compo-nents(with eigenvalues greater than 1)reached 83.501％.Based on the comprehensive evaluation D value,3 high-quality disease-resistant germplasms with higher comprehensive scores(QFY25-3,QFY25-32 and QFY25-8)were selected.关键词
大豆种质资源/抗病基因/变异位点/主成分分析Key words
Glycine max germplasm resources/disease resistance genes/mutation sites/principal component analysis分类
农业科技引用本文复制引用
朱治佳,李敏敏,孙浩月,韩冬伟,张笛,王振,王连霞,何小春,袁明..大豆抗SCN基因rhg1与Rhg4变异位点及育种利用评价[J].种子,2026,45(4):48-55,8.基金项目
黑龙江省农业科技创新跨越工程农业科技基础创新优秀青年项目(CX25JC24) （CX25JC24）
国家现代农业产业技术体系资助项目(CARS-04) （CARS-04）
聊城市重点研发计划政策引导类项目(2024YD91) （2024YD91）
