种子2026，Vol.45Issue(4)：79-87,9.DOI:10.16590/j.cnki.1001-4705.2026.04.079
177份花生种质资源种仁与荚壳的黄曲霉侵染抗性鉴定与评价
Identification and Evaluation for Resistance to Aspergillus flavus Infection in Seeds and Shells of 177 Arachis hypogaea Germplasm Resources
摘要
Abstract
The contamination of Arachis hypogaea with aflatoxin poses a direct threat to the healthy and sustainable development of the Arachis hypogaea industry and significantly restricts China's Arachis hypogaea export trade.To screen specific Arachis hypogaea germplasm resources that are resistant to Aspergillus flavus infection in the seed kernels or pod shells,and to discover excellent materials with dual resistance in both seed kernels and pod shells,the spore fungus suspension infec-tion method was adopted.With 177 Aspergillus flavus germplasm materials as the experimental materials,a systematic identification of Aspergillus flavus infection resistance in seed kernels and pod shells was conducted.The results showed that the infection index of the tested Arachis hypogaea ma-terials in the seed kernels ranged from 1.67 to 96.33,among which 6 were resistant,48 were moder-ately resistant,86 were moderately susceptible,33 were highly susceptible and 4 were highly highly susceptible.The proportion of materials with resistance above the medium level was 30.51％.In the pod shell resistance test,5 highly resistant materials,55 moderately resistant materials,102 moder-ately susceptible materials,and 15 highly susceptible materials were screened out.Among them,Huayu60 showed resistance in the seed kernels and high resistance in the pod shells,being the only excellent germplasm with dual resistance in both seed kernels and pod shells in this study.Correlation analysis indicated that there was no significant correlation between the infection index of Arachis hy-pogaea pods and that of the seed kernels.A total of 6 Arachis hypogaea materials with resistance in the seed kernels,5 highly resistant materials in the pod shells,and 1 material with dual resistance were screened out as excellent Arachis hypogaea materials resistant to Aspergillus flavus.This pro-vided key basic materials for the mining of Aspergillus flavus resistance genes and the genetic improvement of aflatoxin-resistant Arachis hypogaea varieties.关键词
花生/种仁/荚壳/黄曲霉侵染/抗性评价Key words
Arachis hypogaea/seed kernels/shell/Aspergillus flavus infection/resistance evaluation分类
农业科技引用本文复制引用
李梦姣,马俊峰,刘艳丽,王振军,郭振营,张瑞平,赵洋..177份花生种质资源种仁与荚壳的黄曲霉侵染抗性鉴定与评价[J].种子,2026,45(4):79-87,9.基金项目
河南省重点研发专项(251111112700) （251111112700）
河南优势特色农业产业科技支撑行动计划(20230203004) （20230203004）
河南省科技智库调研课题项目(HNKJZK-2025-86B) （HNKJZK-2025-86B）
