浙江医学2026，Vol.48Issue(10)：1016-1020,后插1,6.DOI:10.12056/j.issn.1006-2785.2026.48.10.2025-133
童年期肥胖对实验性自身免疫性脑脊髓炎小鼠抑郁症状及海马小胶质细胞的影响
Effects of childhood obesity on depressive symptoms and hippocampal microglia in experimental autoimmune encephalomyelitis mice
摘要
Abstract
Objective To explore the effects of childhood obesity on depressive-like behaviors and hippocampal microglia in experimental autoimmune encephalomyelitis(EAE)mice.Methods Twenty-four offspring mice delivered by four specific pathogen-free C57BL/6J female mice were selected and divided into observation group and control group by random number table method.All mice were fed separately after weaning at 21 days of age.The observation group was fed with 60％fat-energy diet,while the control group was fed with 10％fat-energy diet,and the EAE model was established at the age of 12 weeks.Sucrose preference test and forced swimming test were performed to evaluate depressive-like behaviors.Immunofluorescence assay was used to detect and analyze the soma projection area,branching index of microglia,as well as the relative fluorescence intensity of inducible nitric oxide synthase(iNOS)and CD206.Enzyme-linked immunosorbent assay(ELISA)was adopted to determine the relative concentrations of inflammatory factors tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in hippocampal tissues.Results The body weight of the observation group at the age of 6,9 and 12 weeks was higher than that of the control group,with statistically significant differences(all P＜0.05).The sucrose preference rate of the observation group was lower than that of the control group,and the immobility time in forced swimming test was longer than that of the control group,with statistically significant differences(all P＜0.05).The relative fluorescence intensity of iNOS in the observation group was higher than that in the control group,while the relative fluorescence intensity of CD206 was lower than that in the control group,with statistically significant differences(all P＜0.05).The expression level of TNF-α and IL-1β in hippocampal tissues of the observation group were higher than those of the control group,with statistically significant differences(all P＜0.05).Conclusion Childhood obesity can affect the function and inflammatory response of hippocampal microglia,and aggravate depressive-like behaviors in EAE mice.关键词
多发性硬化/肥胖/小胶质细胞/抑郁/海马Key words
Multiple sclerosis/Obesity/Microglia/Depression/Hippocampus引用本文复制引用
罗浩源,柴嘉擎,王赵伟..童年期肥胖对实验性自身免疫性脑脊髓炎小鼠抑郁症状及海马小胶质细胞的影响[J].浙江医学,2026,48(10):1016-1020,后插1,6.基金项目
浙江省医坛新秀培养计划项目(YTXX202201) （YTXX202201）
浙江省基础公益研究计划项目(LGD22H090016) （LGD22H090016）
绍兴市卫生健康科技计划项目(2022KY015) （2022KY015）
