浙江医学2026，Vol.48Issue(10)：1025-1030,后插1,7.DOI:10.12056/j.issn.1006-2785.2026.48.10.2025-377
雷公藤甲素减轻心肌细胞缺血再灌注损伤的研究
Triptolide attenuates myocardial ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effect and mechanism of triptolide(Tri)in alleviating ischemia/reperfusion(I/R)injury in cardiomyocytes.Methods H9c2 rat cardiomyocytes were divided into the I/R group,the I/R+Tri group,and the control group.The myocardial enzyme markers reflecting H9c2cell injury,including creatine kinase(CK),creatine kinase isoenzyme(CK-MB),and lactate dehydrogenase(LDH),as well as the indicators reflecting oxidative stress levels in H9c2 cells,including reactive oxygen species(ROS),malondialdehyde(MDA),and superoxide dismutase(SOD),were detected by ELISA.The expression levels of apoptosis-related genes(Bax,p53,Bcl-2,and Caspase-3)were determined by quantitative real-time polymerase chain reaction(qPCR),and the expression levels of apoptosis-related proteins[Bax,Bcl-2,Caspase-3,and cleaved Caspase-3(c-Caspase-3)]were measured by Western blot analysis.Results The levels of CK and LDH in the I/R group and the I/R+Tri group were higher than those in the control group,and the level of ROS in the I/R group was higher than those in the I/R+Tri group and the control group,with statistically significant differences(all P＜0.01).The relative expression levels of Bax,Caspase-3,c-Caspase-3 and the Bax/Bcl-2 ratio in the I/R group were higher than those in the I/R+Tri group and the control group,while the relative expression level of Bcl-2 in the I/R group was lower than those in the I/R+Tri group and the control group.The relative expression levels of Bax,Caspase-3,c-Caspase-3 and the Bax/Bcl-2 ratio in the I/R+Tri group were lower than those in the control group,and the relative expression level of Bcl-2 in the I/R+Tri group was higher than that in the control group,with statistically significant differences(all P＜0.01).Conclusion Tri can alleviate cardiomyocyte I/R injury by reducing oxidative stress and inhibiting mitochondria-related apoptotic factors.关键词
雷公藤甲素/缺血再灌注损伤/氧化应激/线粒体/凋亡Key words
Triptolide/Ischemia-reperfusion injury/Oxidative stress/Mitochondria/Apoptosis引用本文复制引用
赵圣刚,石晓雯,刘磊,李扬,张冉,韩集波,蒋芬芬,李澈,许建江..雷公藤甲素减轻心肌细胞缺血再灌注损伤的研究[J].浙江医学,2026,48(10):1025-1030,后插1,7.基金项目
嘉兴市科技计划项目(2023AY40033) （2023AY40033）
嘉兴市卫生科技计划项目(WKD25003) （WKD25003）
浙江省医药卫生科技计划项目(2019ZD055) （2019ZD055）
