中兽医医药杂志2026，Vol.45Issue(3)：61-67,7.DOI:10.13823/j.cnki.jtcvm.2026.027
西洋参须粗多糖体外免疫活性及抗氧化活性研究
Antioxidant and immunoenhancing activities of polysaccharides from Panax quinquefolium L.root hairy in vitro
摘要
Abstract
To investigate the in vitro biological activity of polysaccharides from Panax quinquefolium L.root hairy,five polysaccharide components(PQLP40,PQLP50,PQLP60,PQLP70,PQLP80)were prepared by using different concentrations of ethanol fractionation precipitation,and in vitro immune enhancement and antioxidant activities were evaluated.The effect of each component on the proliferation of RAW 264.7 macrophages was determined by the MTT assay to assess its immune activity;its antioxidant activity was evaluated by measuring its scavenging ability against DPPH·radicals,hydroxyl radicals,superoxide anion radicals,and ABTS+·cation radicals.In terms of immunological activity,both PQLP40(62.50 µg/mL)and PQLP70(31.25 µg/mL,125.00 µg/mL,500.00 µg/mL)could significantly promote the proliferation of RAW 264.7 cells.Regarding antioxidant activity,polysaccharides from different fractions exhibited different free radical scavenging strengths:PQLP80 had the strongest scavenging activity for DPPH·and·OH radicals,PQLP40 was most effective against·O2-radicals,and PQLP60 showed the highest scavenging activity for ABTS+·.The study indicates that the alcohol precipitation concentration affects the in vitro immunomodulatory and antioxidant activities of Panax quinquefolius polysaccharides,and there are differences in activity among polysaccharides obtained at different alcohol concentrations.Among them,PQLP40 and PQLP80 demonstrate the best immuno-antioxidant activities.关键词
西洋参须多糖/免疫增强活性/抗氧化活性/体外试验Key words
polysaccharides from Panax quinquefolium L.root hairy/immunoenhancing activity/antioxidant activity/in vitro experiment分类
农业科技引用本文复制引用
侯超群,李佳漪,邱增文,郭利伟,王米..西洋参须粗多糖体外免疫活性及抗氧化活性研究[J].中兽医医药杂志,2026,45(3):61-67,7.基金项目
"十四五"国家重点研发计划项目(2023YFD18026053) （2023YFD18026053）
