中国现代医生2026，Vol.64Issue(13)：42-46,65,6.DOI:10.3969/j.issn.1673-9701.2026.13.009
基于UPLC-Q-Orbitrap-MS与网络药理学研究核桃肉炒补骨脂治疗肾阳虚型抑郁症的作用机制
The mechanism of action of Buguzhi stir-fried with Hetaorou in the treatment of depression of deficiency of kidney yang was studied based on UPLC-Q-Orbitrap-MS and network pharmacology
摘要
Abstract
Objective Based on ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-MS)and network pharmacology,the chemical components and potential mechanism of action of"Buguzhi stir-fried with Hetaorou"in the treatment of depression of deficiency of kidney yang were systematically analyzed.Methods The chemical composition of the extract of Buguzhi stir-fried with Hetaorou was identified by UPLC-Q-Orbitrap-MS.The active ingredients and their action targets were screened through network pharmacology methods,and a"component-target-pathway"network was constructed.The biological process and signaling pathways of its treatment of depression were explored through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Results A total of 38 chemical components were identified from Buguzhi stir-fried with Hetaorou,such as psoralen,isopsoralen,and bavachinin.Network pharmacology screening identified 30 active components,117 intersection targets,and 20 key pathways,involving neuroactive ligand-receptor interaction,5-hydroxytryptamine synapse,phosphoinositide 3-kinase/protein kinase B signaling pathway,etc.Key active components included neobavaisoflavone,psoralen,isopsoralen,etc.Conclusion Buguzhi stir-fried with Hetaorou may exert therapeutic effects on depression of deficiency of kidney yang through multi-component,multi-target,and multi-pathway synergistic actions,regulating mechanisms such as neurotransmitters,anti-inflammation,antioxidation and neuroprotection.关键词
核桃肉/补骨脂/抑郁症/UPLC-Q-Orbitrap-MS/网络药理学Key words
Hetaorou/Buguzhi/Depression/UPLC-Q-Orbitrap-MS/Network Pharmacology分类
医药卫生引用本文复制引用
赵权,张卫东,刘产明,朱月琴,黄玮,张晶,王访,陈薇,华丰..基于UPLC-Q-Orbitrap-MS与网络药理学研究核桃肉炒补骨脂治疗肾阳虚型抑郁症的作用机制[J].中国现代医生,2026,64(13):42-46,65,6.基金项目
全国老药工传承工作室建设项目(国中医药人教函[2024]255号) （国中医药人教函[2024]255号）
江苏省老药工传承工作室建设项目(苏中医科教[2024]4号) （苏中医科教[2024]4号）
常州市科技计划项目(CJ20239042) （CJ20239042）
常州市中医医院科研基金资助项目(QN202309) （QN202309）
