植物学报2026，Vol.61Issue(3)：428-436,9.DOI:10.11983/CBB25085
WRI1基因在拟南芥幼苗耐热中的功能
Function of WRI1 in Heat Stress of Arabidopsis Seedlings
摘要
Abstract
INTRODUCTION:Heat stress severely impairs plant growth and crop productivity.WRINKLED1(WRI1),an AP2/EREBP-class transcription factor in Arabidopsis thaliana,orchestrates carbon partitioning between glycolysis and fatty acid bio-synthesis,playing pivotal roles in development and stress adaptation.Elucidating its molecular function under high-temperature stress is critical for improving thermotolerance in crops. RATIONALE:While WRI1's metabolic regulatory function has been elucidated,its role in heat response remains unex-plored.To decipher the molecular mechanism of WRI1-mediated thermotolerance,we integrated genetic approaches(wild type,wri1-4,and WRI1-OE(overexpression))with qRT-PCR and phenotyping under controlled heat stress. RESULTS:In this study,histochemical GUS staining of pWRI1::GUS transgenic lines demonstrated constitutive WRI1 expression throughout Arabidopsis seedlings,with significantly enhanced transcription in cotyledons under heat stress(HS)(P＜0.05).Prolonged HS induced gradual transcriptional attenuation,though the transcription levels remained ele-vated than under the optimal temperature(22 ℃).qRT-PCR confirmed thermo-responsive WRI1 upregulation(peak:1 h HS,3-fold induction),followed by threshold-dependent decline,indicating acute early-phase responsiveness.Endoge-nous immunoassays revealed reduced WRI1 protein accumulation under HS,suggesting HS-impaired protein stability or post-translational regulatory mechanisms.Thermotolerance phenotyping of WT,wri1-4,and WRI1-OE lines showed su-perior HS survival rate in WRI1-OE,with acquired thermotolerance exceeding basal thermotolerance across genotypes,confirming WRI1-mediated positive thermoregulation.The survival rate of WRI1-OE seedlings reached approximately 75％-85％,whereas that of wild type and complementary lines was less than 10％.We found that WRI1-OE reduced reactive oxygen species(ROS)accumulation,where direct transcriptional regulation of HSF/HSP genes(e.g.,HSFA2,HSP101)was excluded by qRT-PCR.Nevertheless,differential gene expression across genotypes indicated WRI1's auxiliary role in thermotolerance via ROS scavenging and indirect proteostasis maintenance. CONCLUSION:This study proved WRI1 as a master regulator of thermotolerance,functioning through synergistic acti-vation of chaperone networks(HSFA2-HSPs)and ROS scavenging.The discovery of its cross-pathway coordination mechanism provides novel insights into plant thermal adaptation,and positions WRI1 as a potentially useful target for breeding climate-resilient crops.关键词
拟南芥/WRI1/热胁迫/基因表达Key words
Arabidopsis/WRI1/heat stress/gene expression引用本文复制引用
古旭雅,林张曼,胡思源,李雪宁,覃晓琳,吴政熹,李诺,冯铭茵,黄瑞华..WRI1基因在拟南芥幼苗耐热中的功能[J].植物学报,2026,61(3):428-436,9.基金项目
国家自然科学基金(No.32200276)和广东省基础与应用基础研究(No.2025A1515011235,No.2023A1515011841) （No.32200276）
