安徽农业科学2026，Vol.54Issue(11)：32-35,4.DOI:10.3969/j.issn.0517-6611.2026.11.007
小果蔷薇花粉发育细胞学观察及月季杂交研究
Cytological Observation of Pollen Development in Rosa cymosa and Research on Its Hybridization with Rose
摘要
Abstract
The pollen development of Rosa cymosa was observed using cytology and hybridization experiments were conducted with three mod-ern roses.Paraffin method revealed that the newly formed microspore tetrads of R.cymosa are enveloped by callose.As the surrounding cal-lose degrades,polygonal microspores(uninucleate pollen)are released.Subsequently,spherical uninucleate peripheral microspores was formed at the peripheral stage after vacuolization.These uninucleate peripheral microspores then undergo an unequal mitotic division,giving rise to a large vegetative cell and a small generative cell.During the development of the bicellular pollen,the generative cell detaches from the pollen wall and integrates into the cytoplasm of the vegetative cell,forming a"cell within a cell"structure.Abnormal pollen grains,in which the generative cell fails to enter the vegetative cell,were observed in the anthers of open flowers,accounting for 16.3%of the total.The ma-ture pollen grain is of the bicellular type.For hybrid pollination of R.cymosa,flower buds with a diameter of 0.45 cm and at the initial slitting stage of the sepals should be collected.When R.cymosa was used as the pollen parent in crosses with three modern rose cultivars"Hi-ohgi","Pink Fan",and"Golden Scepter"),no hybrid seeds were obtained.关键词
小果蔷薇/花粉/细胞学观察/月季/杂交育种Key words
Rosa cymosa Tratt./Pollen/Cytological observation/Rose/Hybridization分类
农业科技引用本文复制引用
乐龙胜,李铭康,刘健伟,夏快飞,温志,伍成厚..小果蔷薇花粉发育细胞学观察及月季杂交研究[J].安徽农业科学,2026,54(11):32-35,4.基金项目
广州市部门预算项目(穗财编[2020]94号,穗财编[2022]9号). （穗财编[2020]94号,穗财编[2022]9号）
