草业科学2026，Vol.43Issue(4)：997-1009,13.DOI:10.11829/j.issn.1001-0629.2025-0021
棉粕和木薯粉混合发酵乳酸菌株的筛选及其对绵羊瘤胃体外发酵的影响
Screening of lactic acid bacteria strains in the mixed fermentation of cottonseed meal and cassava meal and their effect on in vitro rumen fermentation in sheep
摘要
Abstract
This study aimed to screen lactic acid bacteria(LAB)strains for fermenting cottonseed meal(CSM)and to optimize the fermentation process through the addition of cassava meal,thereby enhancing its potential as sheep feed.A total of 48 strains were isolated from various sources,including pickled vegetables,Jiangshui fermented grain slurry,yogurt,and sheep rumen fluid,and seven LAB strains were identified via 16S rDNA sequencing.The growth rates of the different LAB strains were compared,and a factorial experimental design was employed to investigate the effects of different LAB strains and ratios of CSM to cassava meal on sensory indicators,pH,ammonia nitrogen,crude protein,and the gossypol degradation rate in fermented CSM.This was conducted to determine the optimal strain and CSM-to-cassava meal ratio.Additionally,in vitro rumen gas production tests were conducted to assess the rumen fermentation parameters of a total mixed ration for sheep,using the fermented feed as the primary protein source.Based on multiple fermentation evaluation indicators,a CSM-to-cassava meal ratio of 8꞉2 achieved the highest gossypol degradation and favorable fermentation efficiency.Among the strains,one Lactobacillus paracasei strain isolated from yogurt exhibited high free gossypol degradation,rapid growth,lower post-fermentation pH,and suitable ammonia nitrogen and crude protein levels,making it the optimal strain for fermenting CSM in this experiment.The in vitro rumen gas production results showed that the 72-hour gas production of the fermented CSM group and the fermented CSM+cassava meal group were significantly higher than that of the control group(P<0.05).Additionally,the pH of the fermented CSM+cassava meal group was significantly lower than that of the fermented CSM group(P<0.05).This study demonstrates that,with a CSM-to-cassava meal ratio of 8꞉2,fermentation with the L.paracasei strain effectively degrades gossypol,improves CSM quality,and enhances nutrient degradation in the sheep rumen,providing a theoretical basis and practical guidance for the production application of fermented feed.关键词
发酵棉粕/木薯粉/乳酸菌/游离棉酚/羊Key words
fermented cottonseed meal/cassava meal/lactic acid bacteria/free gossypol/sheep引用本文复制引用
柳振,田文锦,李冲,许浩宇,高启浩,贾家乐,徐云飞,年芳,王国秀,李宝胜..棉粕和木薯粉混合发酵乳酸菌株的筛选及其对绵羊瘤胃体外发酵的影响[J].草业科学,2026,43(4):997-1009,13.基金项目
中央引导地方科技发展资金项目(23ZYQC0304) （23ZYQC0304）
甘肃省技术创新引导计划项目东西协作专项(24CXNH010) （24CXNH010）
甘肃农业大学伏羲青年英才计划(Gaufx-04Y01) （Gaufx-04Y01）
