草业学报2026，Vol.35Issue(6)：24-34,11.DOI:10.11686/cyxb2025262
紫花苜蓿新品系对盐胁迫的响应
Responses of new alfalfa lines to salt stress
摘要
Abstract
In this study,we explored the responses of five new alfalfa(Medicago sativa)lines(P1,P2,P3,P4,and P5)and M.sativa cv.'Zhongmu'No.1(ZM)to salt stress.The morphological and physiological parameters of alfalfa in the early flowering stage were measured at 0(CK),8,and 20 days under salt stress(200 mmol·L-1 NaCl).The results show that with the extension of salt stress,there was increasing inhibition of plant height,leaf area,aboveground biomass,underground biomass,and K+content of aboveground and underground parts of alfalfa plants.Salt stress also inhibited the growth of the root system of alfalfa plants,with negative effects on the total root length,total root surface area,average root diameter,root volume,and root tip number.The total flavonoid content and the activities of phenylalanine ammonia-lyase,cinnamic acid-4-hydroxylase,and 4-coumarate-coenzyme A ligase were increased under salt stress,as were the Na+contents in the aboveground and underground parts.Principal component analysis revealed that underground K+and Na+contents,root tip number,plant height,and leaf area are the preferred indices for evaluating the salt tolerance of alfalfa at the early flowering stage.On the basis of a comprehensive evaluation with the membership function method,the M.sativa lines were ranked from most to least salt tolerant as follows:P3>P4>P5>ZM>P2>P1.These findings provide a theoretical basis for breeding new salt-resistant alfalfa cultivars.关键词
紫花苜蓿/耐盐性/黄酮/苯丙氨酸解氨酶Key words
alfalfa/salt tolerance/flavones/phenylalanine ammonia-lyase引用本文复制引用
任孟雨,王利群,南丽丽,郭佳雨..紫花苜蓿新品系对盐胁迫的响应[J].草业学报,2026,35(6):24-34,11.基金项目
甘肃省重大专项(25ZDNA005)资助. （25ZDNA005）
