草业学报2026，Vol.35Issue(6)：155-165,11.DOI:10.11686/cyxb2025254
新疆喀什盐碱地田菁根瘤中菌株分离及其促生效应
Isolation and growth-promoting effects of rhizobia from the Sesbania cannabina seed trial area in saline-alkali land in Kashi,Xinjiang
摘要
Abstract
The aim of this work was to expand microbial fertilizer strain resources and develop rhizobial agents suitable for Sesbania cannabina cultivation in saline-alkali soils.To this end,Rhizobium strains were isolated from the root nodules of S.cannabina growing in a trial planting area in saline-alkali land in Kashi,Xinjiang.The isolates were identified based on 16S rRNA gene sequences amplified by polymerase chain reaction,and by phylogenetic analyses.The effects of the isolated strains,alone or in mixed combinations,on the growth of S.cannabina and the properties of salt-alkali soil were determined.A total of 17 strains were isolated,belonging to the genera Ensifer,Enterobacter,and Brucella,with Ensifer being the dominant genus.The strains Ensifer sp.B-3-1,Ensifer sp.C-3-2,Enterobacter sp.A-5-1,and Brucella sp.C-4-1 exhibited the ability to solubilize both organic and inorganic phosphorus.Inoculation of Ensifer as single strains or mixed with other strains positively affected the growth of S.cannabina;the plant height,stem diameter,aboveground dry weight,chlorophyll content,and root dry weight were increased by 21.2%-56.2%,22.3%-67.8%,4.5%-43.5%,26.8%-149.5%,and 18.2%-100.0%,respectively,and the number of root nodules was also significantly increased(P<0.05).The properties of salt-alkali soil were also improved by Ensifer strains,alone or in combinations;the pH value and total salt,total phosphorus,and total potassium contents were decreased by 2.4%-4.1%,23.6%-39.1%,23.3%-69.4%and 2.2%-21.2%,respectively,and the available nitrogen and available phosphorus contents were increased by 63.5%-98.4%and 0.1%-9.0%,respectively.After inoculation with Ensifer sp.B-3-1,the available potassium content in soil was lower than that of the control group,but inoculation with other Ensifer strains increased the available potassium content in soil by 8.4%-99.7%.Among the various tested combinations,the combination E(Ensifer sp.C-3-2,Ensifer sp.B-3-1,and Brucella sp.C-4-1)had the best effect.The strains re-isolated after inoculation were highly homologous to the inoculated strains B-3-1 and C-3-2,demonstrating their successful colonization of the host.The Rhizobium strains obtained in this study have potential applications in developing microbial fertilizers suitable for reclamation and ecological restoration of saline-alkali land.关键词
根瘤菌/田菁/盐碱地/剑菌属/微生物肥料Key words
rhizobia/Sesbania cannabina/saline-alkali land/Ensifer/microbial fertilizers引用本文复制引用
刘媛媛,杨俊锋,崔棋雯,李明源,王继莲..新疆喀什盐碱地田菁根瘤中菌株分离及其促生效应[J].草业学报,2026,35(6):155-165,11.基金项目
喀什地区科技计划项目(KS2024001),国家自然科学基金(32160408)和喀什大学高层次人才科研启动经费项目(GCC2023ZK-001)资助. （KS2024001）
