大豆科学2026，Vol.45Issue(3)：49-56,8.DOI:10.11861/j.issn.1000-9841.2026.03.0049
大豆GmAP2-35两种可变剪切转录本的盐碱胁迫功能差异及机制
Alternative Splicing of Soybean Gene GmAP2-35 Affects Salt-alkali Tolerance in Soybean
摘要
Abstract
Soybean(Glycine max)is an important food and oil crop,and its yield and quality are often severely restricted by salt-alkali stress.To determine whether alternative splicing of the GmAP2-35 member of the soybean AP2 transcription factor family leads to differences in salt-alkali stress responses,two splicing transcripts(GmAP2-35-1 and GmAP2-35-2)were identified and their functions under salt-alkali stress were compared.By constructing overexpression vectors of the two transcripts and obtaining transgenic soybean lines,they were subjected to salt-alkali treatment,and phenotypes were observed,growth indicators were statistically analyzed,and antioxidant-related physiological and biochemical indicators were measured to analyze the response differences of the different transcripts.The results showed that GmAP2-35-2 was significantly induced under salt-alkali stress,while the expression level of GmAP2-35-1 did not change significantly;the transgenic lines overexpressing GmAP2-35-2 maintained a better growth state under salt-alkali stress,with higher plant height and fresh weight than the GmAP2-35-1 lines,and their SOD(superoxide dismutase),POD(peroxidase),and CAT(catalase)activities were significantly enhanced,while MDA(malondialdehyde)content was significantly reduced and ROS(Reactive Oxygen Species)accumulation was less.The results indicated that alternative splicing of GmAP2-35 led to functional differentiation in salt-alkali stress responses,with GmAP2-35-2 playing a positive regulatory role in enhancing soybean salt-alkali stress tolerance.This study revealed the mechanism by which alternative splicing of AP2 transcription factors functions in soybean's tolerance to adverse conditions,providing new molecular evidence for a deeper understanding of how soybean finely regulates its response to salt-alkali stress through alternative splicing,and offering new research targets for conducting salt-alkali-tolerant molecular breeding using specific transcripts.关键词
大豆/AP2转录因子/可变剪切/盐碱胁迫/抗氧化系统Key words
soybean/AP2 transcript factor/alternative splicing/saline-alkaline stress/antioxidant system引用本文复制引用
李娜,王梅子,徐振宇,房庆喜,韩冬伟,孟凡立..大豆GmAP2-35两种可变剪切转录本的盐碱胁迫功能差异及机制[J].大豆科学,2026,45(3):49-56,8.基金项目
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-B019) （CZKYF2024-1-B019）
黑龙江省自然科学基金项目(JQ2023C008) （JQ2023C008）
中国科学院东北地理与农业生态研究所创新团队项目(2023CXTD01) （2023CXTD01）
吉林省高新技术产业化项目(2024SYHZ0050). （2024SYHZ0050）
