大学化学2026，Vol.41Issue(5)：70-78,9.DOI:10.12461/PKU.DXHX202511031
一种聚集诱导发光荧光探针的合成及其在指纹显影中的应用
Synthesis of an aggregation-induced emission fluorescent probe and its application in fingerprint development:exploration of a comprehensive chemistry experiment for undergraduates
摘要
Abstract
Aggregation-induced emission(AIE)materials represent a cutting-edge system in fluorescence sensing and bioimaging.Introducing these materials into undergraduate laboratory courses holds significant pedagogical value for cultivating innovative chemistry talents.This experiment designed and synthesized an AIE fluorescent probe(TPE-Th-1-OH)based on a tetraphenylethylene(TPE)framework.The target molecule was selected through density functional theory(DFT)calculations and synthesized via Knoevenagel condensation.Structural confirmation was achieved through Proton Nuclear Magnetic Resonance spectroscopy and melting point determination,systematically training students in computational chemistry,organic synthesis,and structural characterization.Photophysical studies demonstrated the probe's excellent fluorescence stability across a wide pH range(3.0-10.0),with intensity variations below 10%.When applied for fingerprint visualization on various substrates(cardboard,basswood,alumina ceramic,acrylic plate,cover glass,and aluminum foil),the probe exhibited high resolution and strong anti-interference capabilities against complex backgrounds.This experiment effectively integrates AIE research with undergraduate teaching,encompassing four key modules:computational chemistry,organic synthesis,spectral characterization,and practical applications.The established"theory-synthesis-characterization-application"integrated teaching system significantly enhances senior undergraduates'comprehensive analytical skills and research innovation capabilities.关键词
聚集诱导发光/荧光探针/四苯乙烯衍生物/指纹显影/综合性化学实验Key words
Aggregation-induced emission/Fluorescent probe/Tetraphenylethylene derivative/Fingerprint development/Comprehensive chemistry experiment分类
社会科学引用本文复制引用
苏宇彬,姚晨宇,陈舒妍,许丽莎,彭敏,王雅雯,彭羽,郑剑峰..一种聚集诱导发光荧光探针的合成及其在指纹显影中的应用[J].大学化学,2026,41(5):70-78,9.基金项目
西南交通大学2024年本科教育教学研究与改革项目 （）
国家级大学生创新创业训练计划项目(202510613091) （202510613091）
