吉林医药学院学报2026，Vol.47Issue(3)：161-166,6.
紫草素诱导肝癌细胞凋亡和自噬效应及其对ROS水平的影响
Shikonin Induces Apoptosis and Autophagy in Liver Cancer Cells and Its Ef-fect on ROS Levels
摘要
Abstract
Objective To explore the mechanism of action of purpurin in inhibiting liver cancer cell line SMMC-7721.Methods SMMC-7721 cells were cultured in vitro and treated with different concentrations of shikonin.The inhibitory rate of relative cell viability was as-sessed using the MTT assay after 72 hours;cell survival rate was evaluated via trypan blue exclusion assay after 24 hours;flow cytometry was employed to analyze apoptosis rate,ROS levels,autophagy activity,and cell cycle distribution in groups treated with different concen-trations of shikonin.Results Shikonin inhibited relative cell viability in a concentration-dependent manner(IC₅₀=1.25 mg/L).Com-pared with the control group,the early apoptosis rate significantly increased in the 0.25 mg/L group(26.89%vs.1.87%,P<0.05);the mean autophagy fluorescence intensities in the 1 and 4 concentration groups were 12.63%and 11.78%,respectively(control group:7.96%,P<0.05).The proportion of viable cells in the 4 concentration group decreased from 95.59%to 42.45%.ROS levels significantly increased in the 1 mg/L and 4 mg/L groups(P<0.05).Treatment with 4 mg/L shikonin increased the proportion of SubG ₁ phase cells(52.22%)and decreased the proportions of S phase and G ₂/M phase cells(P<0.01).Conclusion Shikonin can inhibit the relative vi-ability of hepatocellular carcinoma cells and induce apoptosis and autophagy,effects that may be related to increased ROS levels.关键词
紫草素/肝癌/活性氧/凋亡/自噬Key words
shikonin/liver cancer/reactive oxygen species/apoptosis/autophagy分类
医药卫生引用本文复制引用
王悦玲,姚鹏宇,李瑞祺,安世维,孙美琦,马畅..紫草素诱导肝癌细胞凋亡和自噬效应及其对ROS水平的影响[J].吉林医药学院学报,2026,47(3):161-166,6.基金项目
吉林省大学生创新创业训练项目(S202413706034) （S202413706034）
吉林省卫健委科技能力提升项目(2025WS-KB003) （2025WS-KB003）
