吉林医药学院学报2026，Vol.47Issue(3)：167-172,177,7.
基于ERK/BMP-2/Smad信号通路探讨骨松强骨丸调控骨质疏松症大鼠成骨细胞分化的分子机制
Molecular Mechanism of Gusong Qianggu Pill in Regulating Osteoblast Differ-entiation in Osteoporotic Rats Based on ERK/BMP-2/Smad Signaling Pathway
摘要
Abstract
Objective To investigate the molecular mechanism by which Gusong Qianggu Wan(GSQGW)regulates osteoblast differentia-tion and improves osteoporosis in rats.Methods Forty SPF-grade female Sprague-Dawley(SD)rats were randomly divided into the Sham group,Model group,GSQGW-L group(5 g/kg),and GSQGW-H group(10 g/kg).The body weight,lumbar bone mineral density(BMD),type I collagen(COL-1)in bone tissue,as well as the mRNA and protein expressions of related factors were detected in each-group.Results After 8 weeks of intervention,compared with the Sham group,the Model group showed a significant increase in body weight and a significant decrease in BMD(P<0.05).Compared with the Model group,the BMD of rats in the GSQGW-L and GSQGW-H groups was significantly increased,and the BMD in the GSQGW-H group was higher than that in the GSQGW-L group(P<0.05).Immu-nohistochemical results showed that the Sham group had strong positive expression of COL-1 in bone tissue,with a large area of brown-yellow staining and deep color;the positive expression in the Model group was significantly weakened;the positive expression of COL-1 in the GSQGW-L and GSQGW-H groups was stronger than that in the Model group,and the positive integrated optical density(IOD)value in the GSQGW-H group was higher than that in the GSQGW-L group(P<0.05).Compared with the Sham group,the protein ex-pression levels of Runx2,Osterix,ALP,and OCN in the Model group were significantly decreased(P<0.05);compared with the Model group,the protein expression levels of Runx2,Osterix,ALP,and OCN in the GSQGW-L and GSQGW-H groups were significantly in-creased,and the protein expression in the GSQGW-H group was higher than that in the GSQGW-L group(P<0.05).Compared with the Sham group,the mRNA and protein expression levels of ERK1/2,BMP-2,and Smad1/5 in the Model group were significantly decreased(P<0.05);compared with the Model group,the mRNA and protein expression levels of ERK1/2,BMP-2,and Smad1/5 in the GSQGW-L and GSQGW-H groups were significantly increased,and the increase in the GSQGW-H group was more significant(P<0.05).Conclu-sion Gu Song Qiang Gu Wan can activate the ERK/BMP-2/Smad signaling pathway to promote osteoblast differentiation and inhibit os-teoclast activity,thereby improving BMD and bone microstructure in OP rats,with a dose-dependent therapeutic effect.关键词
骨松强骨丸/骨质疏松/成骨细胞/ERK/BMP-2/Smad/骨密度Key words
Gu Song Qiang Gu Wan/osteoporosis/osteoblast/ERK/BMP-2/Smad/bone mineral density分类
医药卫生引用本文复制引用
王辉,李俊青,吴非燃,朱卉敏..基于ERK/BMP-2/Smad信号通路探讨骨松强骨丸调控骨质疏松症大鼠成骨细胞分化的分子机制[J].吉林医药学院学报,2026,47(3):167-172,177,7.基金项目
2022年度河南省医学科技攻关联合共建项目(LHGJ20220252) （LHGJ20220252）
