高师理科学刊2026，Vol.46Issue(5)：60-66,7.DOI:10.3969/j.issn.1007-9831.2026.05.010
杨芽黄素对胰腺癌细胞PANC-1迁移和铁死亡的影响
Effects of tectochrysin on migration and ferroptosis of pancreatic cancer cells PANC-1
摘要
Abstract
Investigated the impact of TEC on the migration and ferroptosis of pancreatic cancer cells PANC-1.The toxicity of TEC on PANC-1 cells was measured by MTT assay,the data were analyzed and grouped.The effects of TEC on cell proliferation and migration was evaluated by the colony formation and Transwell assay.Kits and flow cytometry were used to observe assess the changes of glutathione(GSH),malondialdehyde(MDA),ferrous ion and lipid reactive oxygen species(Lipid ROS)levels in cells.The protein expression of glutathione peroxidase 4(GPX4),Ferritin heavy chain(FTH1),acyl-CoA synthetase long-chain family member 4(ACSL4)were evaluated by Western Blot.Results shows that with increasing TEC concentration,PANC-1 cell viability,colony formation,migration ability,GSH concentration,GPX4 and FTH1 decreased;while MDA,Fe2+concentration,lipid ROS levels,and ACSL4 protein increased.The ferroptosis inhibitor Ferrostatin-1 restored TEC-induced cell death and migration ability,and restored the expression of GPX4,FTH1,and ACSL4 proteins.These results suggest that TEC can induce ferroptosis in PANC-1 cells and inhibit cell migration.关键词
杨芽黄素/胰腺癌/铁死亡/增殖/迁移Key words
tectochrysin/pancreatic cancer/ferroptosis/proliferation/migration分类
生物科学引用本文复制引用
高子涵,张琦,孙瑶,王艳琼,杜茂宁,柴然琪,张琳,杨清竹..杨芽黄素对胰腺癌细胞PANC-1迁移和铁死亡的影响[J].高师理科学刊,2026,46(5):60-66,7.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(145409209) （145409209）
2025年大学生创新创业训练计划项目(Y202510232257) （Y202510232257）
黑龙江省科学院青年创新基金项目(RC2026GY01) （RC2026GY01）
