贵州农业科学2026，Vol.54Issue(6)：9-15,7.DOI:10.3969/j.issn.1001-3601.2026.06.002
姬竹叶石斛叶绿体基因组特征分析
Analysis on Chloroplast Genome Characteristics of Dendrobium odiosum
摘要
Abstract
[Objective]The chloroplast genome characteristics of Dendrobium odiosum was investigated to provide a reference for botanic classification,genetic evolution,development and utilization of D.odiosum.[Method]The chloroplast genome sequence of D.odiosum obtained by high-throughput sequencing and third generation ONT sequencing technology was used to analyze the structure,characteristics and phyletic evolution relationship of the chloroplast genome in D.odiosum.[Result]The D.odiosum chloroplast genome with a typical tetrad structure and 157 312 bp of sequence length contained 116 unigenes,8 rRNAs,38 tRNAs and 70 protein-coding genes.63 detected SSR sites were mainly mono-and di-nucleotides,and the base compositions were mostly A and T repetitive units.There were 18 061 amino acids in cpDNA coding sequence of D.odiosum.The codons of CDS sequence preferred to end with A/U(T)bases.There was a close genetic relationship between D.odiosum,Dendrobium salaccense and Dendrobium loddigesii.There was a distant genetic relationship between D.odiosum and Dendrobium hancockii and a significant difference in the IR/SC boundary between D.odiosum and D.hancockii.[Conclusion]The chloroplast genome of D.odiosum has a typical tetrad structure,and there is a distant genetic relationship between D.odiosum and D.hancockii.It is speculated that D.odiosum is a separate species.关键词
石斛/姬竹叶石斛/细叶石斛/叶绿体基因组/系统进化/高通量测序Key words
Dendrobe/Dendrobium odiosum/Dendrobium hancockii/chloroplast genome/phyletic evolution/high-throughput sequencing分类
农业科技引用本文复制引用
杜致辉,晋宇轩,陈之林..姬竹叶石斛叶绿体基因组特征分析[J].贵州农业科学,2026,54(6):9-15,7.基金项目
国家自然科学基金项目"MEP途径关键限速酶与DITPSs协同调控美花石斛芳香特征的分子机制(32560097)" （32560097）
贵州省基础研究(自然科学)项目"兴义浓香型美花石斛萜烯类花香成分合成机制研究"(黔科合基础-ZK[2023]一般189) （自然科学）
贵州省科技计划项目"园艺植物基因库创新平台建设及利用"(黔科合服企[2022]005) （黔科合服企[2022]005）
贵州省农业科学院2024年省财政科研专项(种质资源专项)"贵州特色园艺作物种质资源收集、创新及利用"(黔农科种质资源[2023]17号) （种质资源专项）
