贵州农业科学2026，Vol.54Issue(6)：121-130,10.DOI:10.3969/j.issn.1001-3601.2026.06.014
三七主根多糖合成相关且应答"拔鸡腿"基因的挖掘
Study on Genes Related to Polysaccharide Synthesis and Response to Drumstick-forming in Panax notoginseng Taproots
摘要
Abstract
[Objective]The genes related to polysaccharide synthesis and response to drumstick-forming in Panax notoginseng taproots were investigated to lay the foundation for studying the molecular mechanism of high polysaccharide content in P.notoginseng taproots with drumstick-forming characteristics and breeding of P.notoginseng varieties with drumstick-formed characteristics.[Method]The polysaccharide and soluble sugar content in taproots of non-drumstick-formed and drumstick-formed P.notoginseng plants with 2 years and 3 years old were determined by 3,5-dinitrosalicylic acid method and sulfuric acid-anthrone method,respectively.The structural genes and transcription factor genes related to polysaccharide synthesis and response to drumstick-formed in taproots of P.notoginseng were identified based on transcriptome sequencing,quantitative real-time PCR and Pearson correlation analysis.[Result]The polysaccharide content in taproots of drumstick-forming P.notoginseng plants with 2 years and 3 years old was 33.04 mg/g and 55.53 mg/g,75.37%and 92.82%significantly higher than that of non-drumstick-formed P.notoginseng plants with 2 years and 3 years old respectively.The soluble sugar content in taproots of drumstick-formed P.notoginseng plants with 2 years and 3 years old was 204.59 mg/g and 210.30 mg/g,14.08%and 22.19%higher than that of non-drumstick-formed P.notoginseng plants with 2 years and 3 years old respectively.The correlation coefficients between polysaccharide content in taproots of drumstick-formed P.notoginseng plants and the transcriptional level of the UDP-D-glucuronate-4-epimerase gene 1(GAE1),glucose-6-phosphate isomerase gene 2(GPI2),sucrose synthase gene 1(SUS1),phosphoglucomutase gene 2(PGM2)and phosphoglucomutase gene 1(PGM1)was 0.500,0.314,0.293,0.172 and 0.044,respectively.The correlation coefficients between polysaccharide content in taproots of drumstick-formed P.notoginseng plants and transcriptional level of AP2/ethylene responsive factor gene 1(AP2/ERF-1)and AP2/ERF-2 was 0.063 and-0.014,respectively.[Conclusion]The polysaccharide content in taproots of drumstick-formed P.notoginseng is higher than that of non-drumstick-formed P.notoginseng very significantly.The structural genes related to polysaccharide synthesis and response to drumstick-forming in P.notoginseng taproots are SUS1,SUS2,PGM1,PGM2,GPI2 and GAE1,and the transcription factor genes are AP2/ERF-1 and AP2/ERF-2.关键词
三七主根/应答"拔鸡腿"/多糖含量/多糖合成相关基因/相关性Key words
Panax notoginseng taproot/response to"drumstick-forming"/polysaccharide content/polysaccharide synthesis-related gene/correlation分类
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宫晓云,赵昶灵,李仕江,赵鹏宇,顾怀珊,徐绍忠,张锐,张应鹏,彭云,习楚琼..三七主根多糖合成相关且应答"拔鸡腿"基因的挖掘[J].贵州农业科学,2026,54(6):121-130,10.基金项目
国家自然科学基金项目"三七药材皂苷含量'拔鸡腿'响应性增高的机理"(82460741) （82460741）
云南省科学技术协会、云南农业大学和西南林业大学联合资助项目"楚雄市农民院士科技服务站"(A3012025014) （A3012025014）
云南省教育厅2026年度科学研究基金项目"多组学鉴定三七地下部皂苷合成相关且应答'拔鸡腿'的基因"(2026Y0456) （2026Y0456）
云南农业大学第十八届创新行动基金项目"三七地下部代谢组对'拔鸡腿'的响应"(20250407073) （20250407073）
