福建林业科技2026，Vol.53Issue(1)：58-66,74,10.DOI:10.13428/j.cnki.fjlk.2026.01.008
基于RNA-Seq测序的濒危植物井冈山杜鹃转录组特征分析
Transcriptome Characteristics of Endangered Plant Rhododendron jingangshanicum Tam based on RNA-Seq Analysis
摘要
Abstract
Rhododendron jingangshanicum Tam is an alpine ornamental plant with high ornamental and application value.It faces sur-vival threats due to habitat destruction and human disturbance.In order to explore the molecular basis of environmental adaptation and thereby enhance the conservation and effective utilization of the germplasm resources.In this study,we performed transcriptome sequencing of R.jingangshanicum Tam samples along an elevational gradient(1068-1787 m).A total of 230768 Unigenes were as-sembled,of which 55.23%(127455)were annotated in the NR database,51605 and 48401 were annotated by GO and KEGG data-bases,respectively.Functional analysis showed that plant-pathogen interactions,MAPK signaling and oxidative phosphorylation were significantly enriched.A total of 34714 DEGs were identified by differential expression analysis.Among them,RJ1345 vs RJ1504 and RJ1068 vs RJ1787 showed high transcriptome variations,while RJ1504 vs RJ1682 showed significantly fewer DEGs(1385),sugges-ting that the response of gene expression to elevation showed non-linear characteristics.A total of 2906 transcription factors were pre-dicted in the transcription factor family,bHLH(9.87%)and MYB-related(8.01%)family members were the most.A total of 67541 SSR sites were detected by SSR locus analysis,mainly A/T(43.59%)and AG/CT(34.31%)repeats.The results of this study en-riched the genetic information of R.jingangshanicum Tam,and provided a theoretical basis for the subsequent research on the analy-sis of species genetic diversity and the formulation of effective genetic resource protection strategies.关键词
井冈山杜鹃/转录组/代谢途径/转录因子/SSRKey words
Rhododendron jingangshanicum Tam/transcriptome/metabolism pathway/transcription factor/SSR分类
农业科技引用本文复制引用
王植亮,王志勇,余义亮,罗瑞宁,李宜鸿,杨波,叶林江..基于RNA-Seq测序的濒危植物井冈山杜鹃转录组特征分析[J].福建林业科技,2026,53(1):58-66,74,10.基金项目
江西省自然科学基金(20224BAB215012) （20224BAB215012）
国家自然科学基金(32260149) （32260149）
景德镇市科技计划项目(2023NY003) （2023NY003）
