黑龙江八一农垦大学学报2026，Vol.38Issue(3)：18-26,51,10.DOI:10.3969/j.issn.1002-2090.2026.03.003
急性冷应激下OGT上调RBM3促进断奶仔猪骨骼肌葡萄糖代谢的作用机制
OGT Upregulates RBM3 and Promotes Skeletal Muscle Glucose Metabolism in Weaned Piglets under Acute Cold Stress
摘要
Abstract
Low temperature is an inevitable major stressor for animals in cold regions of Northeast China,which can cause systemic metabolic disorders and protein synthesis remodeling in the body.As an important cold shock protein,RBM3 is rapidly upregulated upon low-temperature stimulation and further antagonizes the damage induced by cold stress.This study aimed to elucidate the regulatory role of RBM3 in skeletal muscle glucose metabolism and its pro-survival effect in weaned piglets under acute cold stress,and to verify the molecular mechanism by which OGT mediats the upregulation of RBM3.Nine"Duroc×Landrace×Yorkshire"crossbred weaned piglets were randomly divided into normal temperature control group,4 h cold stimulation group and 8 h cold stimulation group.After acute cold stress treatment at 4 ℃,blood samples and skeletal muscle tissues were collected.The serum levels of CORT,CRH,E,NE and blood glucose,as well as the contents of FDP and PA in skeletal muscle were measured by ELISA.Western blotting(WB)was used to detect the protein expression and phosphorylation levels of OGT,RBM3,AKT,GLUT4,PFKFB2,and GSK3β in skeletal muscle.The contents of ATP,ADP,and AMP in skeletal muscle were measured by high-performance liquid chromatography.The results showed that acute cold stress significantly increased serum CORT,CRH,E and NE levels while decreased blood glucose concentration in weaned piglets.The protein expression and phosphorylation levels of OGT,RBM3,AKT,GLUT4,PFKFB2,and GSK3β,as well as the contents of FDP and PA were upregulated in skeletal muscle,whereas no significant differences were observed in ATP,ADP,and AMP contents among groups.In conclusion,acute cold stimulation induces obvious cold stress responses in weaned piglets.The upregulation of RBM3 promotes the phosphorylation and activation of AKT,thereby regulating skeletal muscle glucose metabolic repro-gramming and inhibiting cell apoptosis.OGT may serve as a key regulatory target mediating the rapid upregulation of RBM3.关键词
急性冷应激/断奶仔猪/OGT/RBM3/葡萄糖代谢Key words
acute cold stress/weaned piglets/OGT/RBM3/glucose metabolism分类
农业科技引用本文复制引用
刘洋,吕红明,张旭,李士泽,胡亚婕..急性冷应激下OGT上调RBM3促进断奶仔猪骨骼肌葡萄糖代谢的作用机制[J].黑龙江八一农垦大学学报,2026,38(3):18-26,51,10.基金项目
黑龙江八一农垦大学引进人才科研启动计划项目(XYB202301,XYB202505) （XYB202301,XYB202505）
黑龙江省博士后科学基金资助项目(LBH-Z24299) （LBH-Z24299）
新时代龙江优秀硕士、博士学位论文资助项目(LJYXL2024-048) （LJYXL2024-048）
大庆市指导性科技计划项目(zd-2025-37) （zd-2025-37）
黑龙江八一农垦大学校级大学生创新创业训练项目(XC2024013). （XC2024013）
