黑龙江八一农垦大学学报2026，Vol.38Issue(3)：43-51,9.DOI:10.3969/j.issn.1002-2090.2026.03.006
超声波辅助英国红芸豆抗氧化肽糖基化改性条件的研究
Study on Glycosylation Modification Conditions of Antioxidant Peptides from British Red Kidney Bean Assisted by Ultrasonic Treatment
摘要
Abstract
To enhance the in vitro antioxidant activity of antioxidant peptides from British red kidney bean,ultrasonic-assisted Maillard reaction was adopted for glycosylation modification.Taking total antioxidant capacity and acrylamide contentas indices,single-factor experiments and response surface methodology were used to determine the optimal conditions for ultrasonic-assisted glycosylation.Subsequently,in vitro simulated digestion and in vitro antioxidant activity analysis were performed on the modified products.The results showed that the optimal glycosylation conditions were as follows:antioxidant peptide concentration 25 mg·mL-1,mass concentration ratio of peptide to sugar 1∶2,ultrasonic power 145 W,ultrasonic temperature 68 ℃,anid ultrasonic time of min.Under these conditions,the total antioxidant capacity of the modified product was 2.23±0.03 mmol·g-1,and the acrylamide content was 223.60±8.24 μg·kg-1.After in vitro simulated digestion,the hydroxyl radical scavenging rate and reducing power of the modified product were significantly increased,which were 63.87％and 95.65％higher than those of the unmodified product,and increased by 53.05％and 95.00％compared with those before digestion respectively.This study provides theoretical basis and data support for improving the in vitro antioxidant activity of antioxidant peptides from British red kidney bean via ultrasonic-assisted glycosylation modification.关键词
英国红芸豆抗氧化肽/超声波处理/糖基化改性/体外抗氧化活性/体外模拟消化Key words
antioxidant peptides from British red kidney bean/ultrasonic treatment/glycosylation modification/in vitro antioxidant activity/in vitro simulated digestion分类
农业科技引用本文复制引用
范晓禹,李玉蕊,崔素萍..超声波辅助英国红芸豆抗氧化肽糖基化改性条件的研究[J].黑龙江八一农垦大学学报,2026,38(3):43-51,9.基金项目
国家重点研发计划项目(2017YFD0400201) （2017YFD0400201）
黑龙江省农垦总局科技攻关项目(HKKYZD190710) （HKKYZD190710）
黑龙江八一农垦大学三横三纵支持计划项目(TDJH201906) （TDJH201906）
黑龙江省教育厅2017年度教改项目(SJGY20170446). （SJGY20170446）
