海南医科大学学报2026，Vol.32Issue(11)：815-822,8.DOI:10.13210/j.cnki.jhmu.20250514.001
lncRNA CASC2靶向miR-K11-3p调控内皮细胞的增殖、凋亡、迁移和侵袭
lncRNA CASC2 regulating the proliferation,apoptosis,migration,and invasion of endothelial cells through targeting miR-K11-3p
摘要
Abstract
Objective:The cell line recognized and widely used in Kaposi's sarcoma research is human umbilical vein endotheli-al cells(HUVECs),to explore the molecular mechanisms of long non-coding RNA cancer susceptibility candidate 2(lncRNA CASC2)on the proliferation,apoptosis,migration,and invasion of HUVECs by targeting miR-K11-3p.Methods:qPCR was used to detect the expression level of lncRNA CASC2;the targeting relationship between lncRNA CASC2 and miR-K11-3p was predicted by bioinformatics and verified by double-luciferase assay and qPCR;CCK-8 assay was used to detect cell proliferation;AnnexinV/Propidium Iodide assay was used to detect cell apoptosis rate;scratch assay was used to detect cell migration ability;Transwell assay was used to detect cell invasion ability.Results:The expression level of CASC2 in the pcDNA-CASC2 group was significantly higher than that in the Control group and pcDNA3.1 group(P＜0.01);lncRNA CASC2 negatively regulated the expression of miR-K11-3p(P＜0.01);lncRNA CASC2 inhibited cell proliferation,promoted cell apoptosis,and reduced cell mi-gration and invasion ability(P＜0.01);exogenously overexpressed miR-K11-3p reversed the effects of lncRNA CASC2 on endo-thelial cells(P＜0.01).Conclusion:LncRNA CASC2 can inhibit HUVECs'proliferation,migration and invasion,and promote cell apoptosis by down-regulating the expression of miR-K11-3p.关键词
长链非编码RNA癌易感性候选基因2(lncRNA CASC2)/miR-K11-3p/人脐静脉内皮细胞(HUVECs)/卡波西肉瘤Key words
lncRNA CASC2/miR-K11-3p/HUVECs/Kaposi's Sarcoma分类
医药卫生引用本文复制引用
张静,彭靖淇,刘娜,房新志..lncRNA CASC2靶向miR-K11-3p调控内皮细胞的增殖、凋亡、迁移和侵袭[J].海南医科大学学报,2026,32(11):815-822,8.基金项目
This study was supported by the Natural Science Foundation Project of Xinjiang Uyghur Autonomous Region(2022D01C562) 新疆维吾尔自治区自然科学基金(2022D01C562) （2022D01C562）
