黑龙江八一农垦大学学报2026，Vol.38Issue(3)：69-76,87,9.DOI:10.3969/j.issn.1002-2090.2026.03.009
羊口疮病毒单克隆抗体2A3可变区鉴定及其建模分析
Identification and Modeling Analysis of the Variable Region of Monoclonal Antibody 2A3 against ORFV
摘要
Abstract
Orf,caused by Orf virus(ORFV),is an acute and highly contagious zoonotic disease that is prevalent in pastoral areas worldwide,posing long-term threat to the development of local animal husbandry.Currently,highly effective and practical diagnostic and therapeutic agents against Orf are scarce.In our previous study,the anti-ORFV-B2L monoclonal antibody 2A3 was prepared,but the amino acid compositions of its heavy chain variable region(VH),light chain variable region(VL),and complementarity determining regions(CDRs)remained unknown,which hindered the applied research of 2A3 and the design of its single-chain antibody.In this study,the VH and VL gene sequences of 2A3 were successfully obtained through RT-PCR.The amino acid composition of the variable regions and CDRs was analyzed and determined using IgBLAST and the VBASE2 database,and their spatial structure was presented through homology modeling.The results showed that the VH gene is 342 bp in length,belonging to the IgG1 subtype,and the VL gene is 336 bp in length,belonging to the Kappa type.Compared with the 2E4 monoclonal antibody,its variable regions and CDRs have unique characteristics.Modeling results indicated that the VH and VL of the 2A3 antibody and their CDRs form a complete and stable"pocket"spatial conformation.This study lays the foundation for exploring the molecular mechanism of 2A3's specific binding to ORFV-B2L and for developing single-chain antibodies against ORFV.关键词
羊口疮病毒/单克隆抗体/可变区/互补决定区/同源建模Key words
Orf virus/monoclonal antibody/variable region/complementary determining region/homology modeling分类
农业科技引用本文复制引用
何鑫,李鑫,黄欣,刘鑫宇,黄慧雯,于永忠..羊口疮病毒单克隆抗体2A3可变区鉴定及其建模分析[J].黑龙江八一农垦大学学报,2026,38(3):69-76,87,9.基金项目
国家自然科学基金面上项目(羊传染性脓疱病毒保护性抗原表位筛选及表位作图研究:31172353) （羊传染性脓疱病毒保护性抗原表位筛选及表位作图研究:31172353）
黑龙江省教育厅课题(口蹄疫病毒Ca2+信号通道结构的研究:11551322) （口蹄疫病毒Ca2+信号通道结构的研究:11551322）
黑龙江八一农垦大学博士科研启动基金(BovIFN-γ/IL-2基因共表达蛋白的筛选及生物学活性鉴定:B2010-09) （BovIFN-γ/IL-2基因共表达蛋白的筛选及生物学活性鉴定:B2010-09）
横向课题(干扰素治疗羊口疮试验:2042100035). （干扰素治疗羊口疮试验:2042100035）
