郑州大学学报（医学版）2026，Vol.61Issue(3)：11-15,5.DOI:10.13705/j.issn.1671-6825.2025.05.106
木犀草素对膀胱癌5637细胞活力、焦亡及NLRP3-Caspase-1-焦亡素D通路的影响
Effects of luteolin on viability,pyroptosis,and NLRP3-Caspase-1-gasder-min D pathway of bladder cancer 5637 cells
摘要
Abstract
Aim:To explore the role of luteolin in viability and pyroptosis of bladder cancer cells and its molecular mechanism.Methods:CCK-8 assay was used to detect the viability of 5637 cells after treatment with low,medium,and high doses(25,50,100 μmol/L)of luteolin for 12,24,and 48 hours.5637 cells were divided into 5 groups:normal control group(PBS),positive control group(5 μmol/L pyroptosis inducer nigericin),and low-dose(25 μmol/L),medium-dose(50 μmol/L),and high-dose(100 μmol/L)luteolin groups.After 24 hours of treatment,immunofluorescence staining was used to detect gasdermin D(GSDMD)protein expression in each group.ELISA was performed to measure the secretion levels of lactate dehydrogenase(LDH),IL-18,and IL-1β in the cell supernatant.The 2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)method was used to detect intracellular reactive oxygen species(ROS)levels.Western blot was employed to determine the expression of pyroptosis pathway proteins NLRP3,Caspase-1,GSDMD,and inflammatory cytokines IL-1β and IL-18.Additionally,5637 cells were divided into 3 groups:normal control group,low-dose luteolin group,and pyroptosis in-hibitor group(25 μmol/L luteolin+20 μmol/L Caspase-1 inhibitor VRT-043198),Western blot was used to detect the ex-pression of Caspase-1,GSDMD,IL-1β,and IL-18 after 24 hours of treatment.Results:With increasing luteolin treatment time and dose,the viability of 5637 cells showed a decreasing trend(P＜0.05).Compared with the normal control group,the luteolin dose groups and the positive control group exhibited abundant GSDMD protein aggregation on the cell mem-brane,increased secretion of LDH,IL-1β,and IL-18,indicating induced pyroptosis.Meanwhile,intracellular ROS level in-creased,and the protein expression of NLRP3,Caspase-1,GSDMD,IL-1β,and IL-18 was upregulated(P＜0.05).Further-more,compared with the low-dose luteolin group,treatment with Caspase-1 inhibitor markedly decreased the protein expres-sion of Caspase-1,GSDMD,IL-1β and IL-18 in the pyroptosis inhibitor group.Conclusion:Luteolin may inhibit the viabili-ty of bladder cancer cells by inducing pyroptosis of 5637 cells through activating the NLRP3-Caspase-1-GSDMD pathway.关键词
木犀草素/膀胱癌/焦亡/焦亡素D/5637细胞/NLRP3-Caspase-1-GSDMD通路Key words
luteolin/bladder cancer/pyroptosis/gasdermin D/5637 cell/NLRP3-Caspase-1-gasdermin D pathway分类
医药卫生引用本文复制引用
姚晓含,李赫阳,孙振邦,杨璨宇,王明,韩滨..木犀草素对膀胱癌5637细胞活力、焦亡及NLRP3-Caspase-1-焦亡素D通路的影响[J].郑州大学学报（医学版）,2026,61(3):11-15,5.基金项目
河南省创新研发专项(HNGD2024005) （HNGD2024005）
